GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

22 Samples

Download data: CSV

(Submitter supplied) In this work we compare the molecular functions of Myf5 and MyoD, two highly related bHLH transcription factors that regulate skeletal muscle specification and differentiation. We find MyoD and Myf5 bind the same sites genome-wide but have distinct functions: Myf5 induces histone acetylation without Pol II recruitment or robust gene activation, whereas MyoD induces histone acetylation, recruits PolII and robustly activates gene transcription.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: CSV

(Submitter supplied) In this work we compare the molecular functions of Myf5 and MyoD, two highly related bHLH transcription factors that regulate skeletal muscle specification and differentiation. We find MyoD and Myf5 bind the same sites genome-wide but have distinct functions: Myf5 induces histone acetylation without Pol II recruitment or robust gene activation, whereas MyoD induces histone acetylation, recruits PolII and robustly activates gene transcription.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

10 Samples

Download data: CSV

(Submitter supplied) Gene expression changes induced by MyoD or Myf5 were examined in a double-knockout fibroblast cell line lacking endogenous functional myoD or myf5 genes. Use of this cell line precluded the possibility of auto- or cross-activation of endogenous myoD or myf5. Myogenin or hrGFP were expressed in parallel samples as controls. Following infection with retrovirus - expressing the relevant myogenic regulatory factor (MRF) from the viral LTR promoter and hrGFP through an IRES element in the same mRNA transcript - GFP+ cells were sorted by FACS and harvested for total RNA. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

12 Samples

Download data: CEL, EXP

(Submitter supplied) Muscle satellite cells are a self-renewing pool of stem cells that give rise to daughter myogenic precursor cells in adult skeletal muscle. Published and preliminary data indicated that MyoD and p53 genes are involved in satellite cell differentiation. We would like to know what downstream genes of both transcription factors are affected in satellite cell-derived myoblasts (MyoD-/-, p53 -/-). Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL81 GPL82 GPL83

25 Samples

Download data: CEL, EXP

(Submitter supplied) The transcription factor MyoD can coax na?e fibroblasts or otherwise committed cells to adopt the skeletal muscle phenotype by activating the muscle gene expression program. Activation of muscle gene expression occurs in quantal steps with not all the target genes of MyoD being activated at the same time. Some genes are induced in the initial phases, others at later stages despite the fact that MyoD is present throughout the differentiation process. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2854

Platform:

GPL1261

30 Samples

Download data: CEL

Analysis of embryonic fibroblasts at various time points up to 24 hours following transcriptional induction of a mutant form of the myogenic transcription factor MyoD. MyoD altered at lysine residues 99, 102, and 104, rendering it not acetylatable.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 protocol, 4 time sets

Platform:

GPL1261

Series:

GSE6487

30 Samples

Download data: CEL

(Submitter supplied) We used a combination of genome-wide and promoter-specific DNA binding and expression analyses to assess the functional roles of Myod and Myog in regulating the program of skeletal muscle gene expression. Our findings indicate that Myod and Myog have distinct regulatory roles at a similar set of target genes. At genes expressed throughout the program of myogenic differentiation, Myod can bind and recruit histone acetyltransferases. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2334

Platform:

GPL339

36 Samples

Download data: CEL, EXP

Temporal analysis of embryonic Myf-5/Myod null fibroblasts tranduced with a Myod-estrogen receptor hormone binding domain fusion protein alone or in combination with a constitutively expressed Myog. Results provide insight into the roles of Myod and Myog in muscle differentiation.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 agent, 2 cell line, 4 time sets

Platform:

GPL339

Series:

GSE3858

36 Samples

Download data: CEL, EXP

(Submitter supplied) Transcriptional profiling comparing the genes upregulated or downregulated by active hGR-Xebf3 by treating hormone DEX to those by non-active hGR-Xebf3 in Xenopus animal caps.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10302

2 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling comparing the genes upregulated or downregulated by active hGR-Xebf3 by treating hormone DEX to those by non-active hGR-Xebf3 in Xenopus animal caps.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL11258 GPL10302

8 Samples

Download data: TXT

(Submitter supplied) To identify Pax7 target genes in muscle progenitor cells, we compared the transcriptome profiles of muscle progenitor cells from young mice (P12) with and without Pax7.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11002

2 Samples

Download data: XLS

(Submitter supplied) Pbx homeodomain proteins have been implicated in the regulation of gene expression during muscle development. Whether Pbx proteins are required broadly for the regulation of muscle gene expression or are required for the expression of a specific subset of muscle gene expression is not known. We employed microarrays to determine the requirements for Pbx proteins during zebrafish development. Keywords: developmental time course analysis

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

12 Samples

Download data: CEL

(Submitter supplied) Investigate the genome-wide gene expression profiles of 50% and 95% confluent C2C12 myoblasts and C2C12 myotubes differentiated for 24 and 48 hours.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

12 Samples

Download data: TXT

(Submitter supplied) In an effort to identify the compendium of regulatory elements that govern myogenic differentiation, we generated chromatin state maps based on the recruitment of factors (p300 and PolII) and histone modifications (H3K4me1, H3K27ac) that typify enhancers in myoblasts and myotubes. We found a remarkable concordance between the location of these newly defined, active enhancers and MyoD1 binding events. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9250 GPL13112

14 Samples

Download data: BED, TXT

(Submitter supplied) In order to understand how Sox6 coordinately regulates the transcription of multiple fiber type specific genes during muscle development, we have performed ChIP-seq analyses to identify Sox6 target genes as well as RNA polymerase II (Pol II) binding sites in mouse fetal myotubes.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9250

6 Samples

Download data: BED, GFF

(Submitter supplied) Histone variants complement and integrate histone post-translational modifications in regulating transcription. The histone variant macroH2A1 (mH2A1) is almost three times the size of its canonical H2A counterpart due to the presence of a ~25kDa evolutionarily conserved non-histone macro domain. Strikingly, mH2A1 can mediate both gene repression and activation. However, the molecular determinants conferring these alternative functions remain elusive. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL17021 GPL13112

41 Samples

Download data: BED, BW, TXT

(Submitter supplied) This study describes a cDNA microarray analysis that compared developing mouse MyoD-/- limb musculature (MyoD-dependent, innervated by Lateral Motor Column motor neurons) and Myf5-/- back (epaxial) musculature (Myf5-dependent, innervated by Medial Motor Column motor neurons) to the control and to each other, at embryonic day 13.5 which coincides with the robust programmed cell death of motor neurons and the inability of myogenesis to undergo its normal progression in the absence of Myf5 and MyoD that at this embryonic day cannot substitute for each other. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL1261 GPL9250 GPL11002

33 Samples

Download data: BAM, BEDGRAPH, CEL, MAP

(Submitter supplied) The regulatory networks of differentiation programs have been partly characterized; however, the molecular mechanisms of lineage-specific gene regulation by highly similar transcription factors remain largely unknown. Here we compare the genome-wide binding and transcription profiles of NEUROD2-mediated neurogenesis with MYOD-mediated myogenesis. We demonstrate that NEUROD2 and MYOD bind a shared CAGCTG E-box motif and E-box motifs specific for each factor: CAGGTG for MYOD and CAGATG for NEUROD2. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL9250 GPL11002

9 Samples

Download data: BAM, BEDGRAPH, MAP