GEO DataSets

(Submitter supplied) Histological resolution of the murine pancreas occurs within one week after injury. Whether histological resolution constitutes pancreatic recovery at a molecular level is not known. We performed RNA-sequencing on the recovering pancreas to determine the transcriptomic profile within the histologically recovered pancreas. We show that although there is histological resolution one week after injury in mice, compared to baseline (non-injured pancreas), there are still numerous differentially expressed genes (DEGs) at one and even two weeks after injury. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

23 Samples

Download data: TXT

(Submitter supplied) Upon injury, immune cells undergo dynamic changes in population size, differentiation state and function in response to maintain organismal integrity. Myeloid cells, mostly macrophages have been shown to act as positive regulators of development, tissue homoeostasis, inflammation，especially in inducing resolution after inflammation, remodeling and repair. Here we report what specific role macrophages play in every precisely dynamical period after injury in vivo. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: TXT

(Submitter supplied) TCGA(PAAD) public database and PDAC tissue array with SETD2/H3K36me3 staining was used to investigate the clinical relevance of SETD2 in PDAC. Furthermore, to define the role of SETD2 in the carcinogenesis of PDAC, we crossed conditional Setd2 knockout mice (PdxcreSetd2flox/flox) together with with KrasG12D mice. Moreover, to examine the role of SETD2 after ductal metaplasia, Crisp/cas9 was used to deplete Setd2 in PDAC cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21273

10 Samples

Download data: BED, TXT

(Submitter supplied) BACKGROUND & AIMS: Acinar cells produce digestive enzymes that impede transcriptomic characterization of the exocrine pancreas. Thus, single-cell RNA-sequencing (scRNA-seq) studies of the pancreas underrepresent acinar cells relative to histological expectations, and a robust approach to capture pancreatic cell responses in disease states is needed. We sought to innovate a method that overcomes these challenges to accelerate study of the pancreas in health and disease. more...

Organism:

Mus musculus

Type:

Other

Platform:

GPL24247

57 Samples

Download data: DCC, XLSX

(Submitter supplied) The rapidly aging population has an increased alcohol use, resulting in increased risk and mortality of alcohol-associated acute pancreatitis in the elderly population. We established an aging- and alcohol-associated acute pancreatitis mouse model and applied spatial transcriptomics to profile heterogenous tissue composition in the mouse pancreas and the associated gene expression. By comparing with the young cohorts, our analysis has revealed the key differentially expressed genes in functional tissue compartments for understanding the mechanisms and developing targeted therapeutic strategies.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24247

4 Samples

Download data: CSV, H5, JPG, JSON, PNG

(Submitter supplied) Transcriptome analysis by RNA-seq in pancreas identified 529 differentially expressed genes between wild-type and Sirt2-deficient mice 2 days after injury and confirmed continuous inflammention and tissue regeneration, as well as delayed tissue reorganization.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

9 Samples

Download data: TXT

(Submitter supplied) Mouse pancreas from wild type and MistKO animals were induced either with caerulein or saline as control and processed for RNA. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix Mouse Expression chips 430. Comparisons between the sample groups allow the identification of genes with differential expression patterns of genes which might contribute to pancreatitis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1731

Platform:

GPL339

12 Samples

Download data

Analysis of pancreas from transcription factor Mist1 knockouts (Mist1KO) injected with caerulein. Mist1KOs exhibit disrupted cell morphology in adult pancreatic acini; caerulein induces acute pancreatitis. Results identify genes that may contribute to susceptibility and initiation of pancreatitis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE3644

12 Samples

Download data

(Submitter supplied) Epigenetic profile of tissues is reprogrammed under diseased conditions. H3K4Me3 and H3K27Me3 represent active and repressive epigenetic marks, respectively. ChIP-seq is an effective tool to study global protein-DNA interactions. To study global epigenetic differences, we used H3K4Me3 antibody to evaluate its enrichment in pancreatic acinar cells of WT and Mist1-/- mice followed by next generation sequencing. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9250

4 Samples

Download data: FA, TAR, TXT

(Submitter supplied) In the current study, we characterized the global miRNA expression profile in mouse pancreatic acinar cells during acute pancreatitis using next-generation RNA-Sequencing. We identified 330 known and 6 novel miRNAs being expressed in mouse pancreatic acinar cells. At basal state, miR-148a-3p, miR-375-3p, miR-217-5p, miR-216a-5p were among the most abundantly expressed whereas miR-24-5p and miR-421-3p were least abundant. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL13112

15 Samples

Download data: TXT

(Submitter supplied) A frequently used experimental model of chronic pancreatitis (PC) recapitulating human disease is repeated injection of cerulein to mice. We found that two common substrains of C57BL/6 , C56BL/6J (Jackson) and C57BL/6NHsd (Harlan), exhibit different degree of CP with C57BL/6J beeing more susceptible to repetitive cerulein induced CP. The goal of this study was to identify genes associated with CP and also to identify genes differentially regulated between two substrains as candidates for the CP progression.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL