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| Status |
Public on Jan 23, 2018 |
| Title |
Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Histological resolution of the murine pancreas occurs within one week after injury. Whether histological resolution constitutes pancreatic recovery at a molecular level is not known. We performed RNA-sequencing on the recovering pancreas to determine the transcriptomic profile within the histologically recovered pancreas. We show that although there is histological resolution one week after injury in mice, compared to baseline (non-injured pancreas), there are still numerous differentially expressed genes (DEGs) at one and even two weeks after injury. Overall, the findings suggest the actual recovery takes longer than initially thought given the differential transcriptomic profile in the pancreas two weeks after injury compared to the baseline pancreas. There is also the possibility of a novel emerging pancreatic transcriptome upon recovery.
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| Overall design |
Acute pancreatitis was induced by caerulein hyperstimulation in both male and female C57BL/6 mice. Total RNA was extracted from the head of the murine pancreas in mice at baseline (non-injured; n=8), day 7 (post-injury; n=8), and day 14 (post-injury; n=7). Total stranded RNA libraries (ribo-depleted) were generated and sequenced on the Illumina NextSeq 500 NGS platform. RNA-seq data was analyzed for differentially expressed genes between baseline and day 7 and between baseline and day 14.
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| Contributor(s) |
Husain SZ, Boggs K, Wang T |
| Citation(s) |
29362419 |
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Kristy Boggs, Ting Wang, Abrahim I. Orabi, Amitava Mukherjee, John F. Eisses, Tao Sun, Li Wen, Tanveer A. Javed, Farzad Esni, Wei Chen and Sohail Z. Husain. (2018). Pancreatic gene expression during recovery after pancreatitis reveals unique transcriptome profiles. Scientific Reports, 8(1), 1406. http://doi.org/10.1038/s41598-018-19392-0
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| Submission date |
Jun 07, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Ting Wang |
| E-mail(s) |
wang9ting@gmail.com
|
| Organization name |
Fred Hutchinson Cancer Research Center
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| Department |
Clinical Research
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| Street address |
1100 Fairview Ave. N.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA389578 |
| SRA |
SRP108766 |
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