GEO DataSets

(Submitter supplied) BATF requires the STAT5 signal to mediate plasticity at the Il9 locus.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24247

6 Samples

Download data: BROADPEAK

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL19057 GPL24247

18 Samples

Download data: BROADPEAK

(Submitter supplied) BATF requires the STAT5 signal to mediate plasticity at the Il9 locus.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

12 Samples

Download data: NARROWPEAK

(Submitter supplied) Microarray analyses were performed to compare gene expression in cultured mouse Th9, Th2 and Treg cells and resting versus activated Th9 cells.

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS5291 GDS5343

Platform:

GPL7202

12 Samples

Download data: TXT

Analysis of naive CD4+ T cells cultured under T helper type 9 (Th9) conditions for 5 days and activated with anti-CD3 antibody. Th9 cells are a subpopulation of CD4+ effector T cells that secrete high levels of interleukin-9. Results provide insight into the molecular basis of the Th9 phenotype.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL7202

Series:

GSE44937

6 Samples

Download data: TXT

Analysis of naive CD4+ T cells cultured under T helper type 9 (Th9), Th2, or Treg conditions for 5 days. Th9 cells are a subpopulation of CD4+ effector T cells that secrete high levels of interleukin-9. Results provide insight into the molecular basis of the Th9 phenotype.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 3 cell type sets

Platform:

GPL7202

Series:

GSE44937

9 Samples

Download data: TXT

(Submitter supplied) The TNF family member TL1A (TNFSF15) co-stimulates several T helper subsets and promotes T cell-dependent models of inflammatory diseases, including inflammatory bowel diseases (IBD) and allergic lung disease. TL1A polymorphisms confer susceptibility to IBD and have been associated with disease severity. In this study, we identified TL1A as a strong inducer of TH9 cell differentiation in vitro. Mechanistically, TL1A induced NF-B signaling and down-stream STAT6 activation and facilitated cooperative binding of BATF, BATF3, and IRF4 to the Il9 promoter. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18635

4 Samples

Download data: TXT

(Submitter supplied) Enhancers regulating Th cell specific cytokine gene expression were studied and identified. However, Il9 regulating enhancer was not identified yet. Active enhancers are characterized with P300, STATs binding and active histone modifications such as H3K4me1 and H3K27ac. Based on P300 ChIP seq analysis, we narrowed down enhancer candidates for Il9 gene expression.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

4 Samples

Download data: BEDGRAPH

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL24247

110 Samples

Download data: BED, BW

(Submitter supplied) To investigate changes in gene expression over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production We then performed gene expression profiling analysis using data obtained from RNA-seq in cells from 2 different human donors and from 2 mice at 4 time points.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24247 GPL24676

28 Samples

Download data: TXT

(Submitter supplied) To investigate changes in chromatin accessibility over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production We then performed Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K4me1, K3K4me3, and H3K27Ac in cells from 2 different human donors and from 2 mice at 4 time points.

Organism:

Homo sapiens; Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL24247 GPL24676

64 Samples

Download data: BED

(Submitter supplied) To investigate changes in chromatin accessibility over time, we differentiated Th9 cells in vitro from naïve T cells from human and murine sources, and followed them over an extended time course, during which they gained and then lost capacity for IL-9 production

Organism:

Mus musculus; Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL24247 GPL24676

18 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

62 Samples

Download data

(Submitter supplied) Th17 cells secrete IL-17A, IL-17F, IL-21, and IL-22 cytokines that are critical in mediating inflammation and protecting the host from microorganisms infection. The basic leucine zipper transcription factor ATF-like (Batf) contributes to the transcriptional programming of multiple effector T cells, and is required for Th17 cell development. Here, we have interrogated mechanisms by which Batf promotes and stabilizes Th17 cell phenotype. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: BW

(Submitter supplied) Th17 cells secrete IL-17A, IL-17F, IL-21, and IL-22 cytokines that are critical in mediating inflammation and protecting the host from microorganisms infection. The basic leucine zipper transcription factor ATF-like (Batf) contributes to the transcriptional programming of multiple effector T cells, and is required for Th17 cell development. Here, we have interrogated mechanisms by which Batf promotes and stabilizes Th17 cell phenotype. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

54 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Other

Platform:

GPL17021

109 Samples

Download data

(Submitter supplied) We have examined mechanisms by which Batf acts to initiate gene transcription in developing effector CD4 T cells. We find that in addition to its pioneering function, Batf controls developmentally regulated recruitment of the chromatin architectural factor, Ctcf, to promote chromatin looping that is associated with transcription of lineage-specific genes. The chromatin organizing actions of Batf are largely Ets1-dependent, which appears to be indispensable for the Batf-dependent recruitment of Ctcf. more...

Organism:

Mus musculus

Type:

Other

Platform:

GPL17021

10 Samples

Download data: BEDGRAPH

(Submitter supplied) We have examined mechanisms by which Batf acts to initiate gene transcription in developing effector CD4 T cells. We find that in addition to its pioneering function, Batf controls developmentally regulated recruitment of the chromatin architectural factor, Ctcf, to promote chromatin looping that is associated with transcription of lineage-specific genes. The chromatin organizing actions of Batf are largely Ets1-dependent, which appears to be indispensable for the Batf-dependent recruitment of Ctcf. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

30 Samples

Download data: BEDGRAPH

(Submitter supplied) We have examined mechanisms by which Batf acts to initiate gene transcription in developing effector CD4 T cells. We find that in addition to its pioneering function, Batf controls developmentally regulated recruitment of the chromatin architectural factor, Ctcf, to promote chromatin looping that is associated with transcription of lineage-specific genes. The chromatin organizing actions of Batf are largely Ets1-dependent, which appears to be indispensable for the Batf-dependent recruitment of Ctcf. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

69 Samples

Download data: BEDGRAPH

(Submitter supplied) The transcription factor BATF is required for Th17 and TFH differentiation. Here, we show that BATF also has a fundamental role in regulating effector CD8+ T cell differentiation. BATF-deficient CD8+ T cells show profound defects in effector expansion and undergo proliferative and metabolic catastrophe early after antigen encounter. BATF, together with IRF4 and Jun proteins, binds to and promotes early expression of genes encoding lineage-specific transcription-factors (T-bet and Blimp-1) and cytokine receptors, while paradoxically repressing genes encoding effector molecules (IFNg and granzyme B). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

13 Samples

Download data: CEL