GEO DataSets

Analysis of A549 lung cancer cells following treatment with anti-cancer agent sapphyrin PCI-2050 or transcription inhibitor actinomycin D. Hydrophilic sapphyrins localize to tumors, generate oxidative stress, and inhibit gene expression.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 dose sets

Platform:

GPL570

Series:

GSE6400

12 Samples

Download data: CEL

(Submitter supplied) The goal of this project was to characterize changes in gene expression in response to the anti-cancer agent sapphyrin PCI-2050. Cultured A549 human lung cancer cells were treated with sapphyrin PCI-2050 or actinomycin D, a known transcripitonal inhibitor. The gene expression profiles of drug-treated and control A549 cultures were determined using Human Genome U133 Plus 2.0 Arrays (Affymetrix, Santa Clara, CA). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2499

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) We have demonstrated that water-soluble zinc ionophores can be administered to mice at relatively high doses and inhibit the growth of A549 lung cancer cells grown in xenograft models. Gene expression profiles of tumor specimens harvested from mice four hours after treatment confirmed that the activation of stress responsive genes occurs in vivo. These findings lead us to propose that the pharmacologic delivery of zinc to tumors using water solubilized ionophores is a potential approach to cancer therapy. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) We have shown that water solubilized versions of a zinc ionophore increase intracellular concentrations of free zinc and have antiproliferative activity in exponential phase A549 lung cancer cultures. The gene expression profiles of A549 lung cancer cultures treated with the lead compound PCI-5002 reveal the activation of stress response pathways. Medium supplementation with zinc (25 μM) led to activation of additional oxidative stress response as well as apoptotic pathways. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) HTI-286, an analogue of hemiasterlin, interferes with microtubule dynamics and circumvents transport-based resistance to taxanes. In this study we evaluate the inhibitory effects of HTI-286 on human prostate cancer growth in vitro and in different in vivo models. The results show that HTI-286 is a potent inhibitor of proliferation and induced marked increases in apoptotic rates in all cell lines tested. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2971

Platform:

GPL3877

12 Samples

Download data

Analysis of prostate cancer LNCaP cells treated with the chemotherapeutic agent docetaxel or the hemiasterlin analog HTI-286. Like docetaxel, HTI-286 disrupts microtubule dynamics. But unlike docetaxel, HT-286 exhibits reduced multidrug resistance.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 agent sets

Platform:

GPL3877

Series:

GSE8325

12 Samples

Download data

(Submitter supplied) To assist in the identification of proteases, their endogenous inhibitors, and proteins that interact with proteases or proteolytic pathways in biological tissues, a dual species oligonucleotide microarray has been developed in conjunction with Affymetrix, Inc. To investigate the performance of the array, gene expression profiles were analyzed in pure mouse and human samples and orthotopically implanted xenografts of human A549 lung and MDA-MB-231 breast carcinomas. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platform:

GPL3385

64 Samples

Download data: CEL

(Submitter supplied) To identify molecular changes that occur during prostate tumor progression, we have characterized a series of prostate cancer cell lines isolated at different stages of tumorigenesis from C3(1)/Tag transgenic mice.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20562

19 Samples

Download data: GPR

(Submitter supplied) We report the effect of motexafin gadolinium (MGd) and exogenous zinc on intracellular levels of free zinc, oxidative stress, proliferation, cell cycle, and cell death in exponential phase human B-cell lymphoma and other hematologic cell lines. We find that increased levels of oxidative stress and intracellular free zinc precede and correlate with cell cycle arrest and apoptotic response. Treatment with exogenous zinc increased oxidative stress and, conversely, oxidative stress resulted in increased intracellular free zinc levels. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1617

Platform:

GPL570

18 Samples

Download data

Analysis of Ramos B-cell lymphoma cultures following 4 hour treatment with anticancer agent motexafin gadolinium (MGd), zinc acetate (25 or 50 uM), or combinations of MGd and zinc. Results provide insight into the molecular changes that accompany the disruption of intracellular zinc homeostasis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 6 agent sets

Platform:

GPL570

Series:

GSE2964

18 Samples

Download data

(Submitter supplied) The 60 cell lines of the NCI Anti-Cancer Drug Screen (NCI60) constitute the most extensively characterized in vitro cancer cell model, and have been tested for sensitivity to over 100,000 potential chemotherapy agents. We have used the NCI60 cell lines and three additional lymphoblast lines to develop a database of responses of cancer cells to ionizing radiation. We compared clonogenic survival, apoptosis induction, and gene expression response by microarray analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5080

63 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

98 Samples

Download data: CEL

(Submitter supplied) Basal gene expression levels were determined by global gene expression profiling of breast cancer cell lines. Molecular subtype was determined using gene expression and HER2 status assesses by HER2 FISH analysis. Keywords: Basal gene expression (no stimulation or timecourse)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

51 Samples

Download data: CEL

(Submitter supplied) MCF10A cells were then transfected with MEK1(S217S221), HRAS(G12V), and null control vectors Cells were lysed 24 hours post-transfection with collection of total RNA and protein Keywords: Oncogene inducation of gene expression changes

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

17 Samples

Download data: CEL

(Submitter supplied) We used microarrays to profile 30 human primary breast tumors and determine global gene expression patterns and molecular subtypes Keywords: Basal gene expression in human tumor samples

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

30 Samples

Download data: CEL

(Submitter supplied) Human lung cancer (A549) cells were treated 50uM of the metal cation-containing chemotherapeutic drug motexafin gadolinium (MGd) for 4, 12, and 24 hrs and expression compared to control cells (treated with 5% mannitol for the same length of time) Keywords: dose response

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1204

Platform:

GPL96

18 Samples

Download data: CEL, CHP, EXP, RPT, TXT

Expression profiling of lung cancer cell line A549 following treatment with 50 uM of the metal cation-containing chemotherapeutic drug motexafin gadolinium (MGd). Cells examined at 4, 12, and 24 hours following treatment. Results provide insight into the mechanism of action of MGd.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 time sets

Platform:

GPL96

Series:

GSE2189

18 Samples

Download data: CEL, CHP, EXP, RPT, TXT