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Status
Public on Jun 16, 2006
Title
PRHU05-S1-0006 (PC Human Operon v2 21k)
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organism
Homo sapiens Manufacturer
Microarray Facility - the Prostate Centre at Vancouver General Hospital
Manufacture protocol
This array consists of 21,329 70mer probes representing 21,329 genes ( Operon Version 2.1 of the Human Genome Oligo Set). Oligonucleotides are consolidated into 384-well plates, lyophilized by speed-Vac, and resuspended in 3x SSC to a printing concentration of 30uM. Oligos are printed on aminosilane slides (Erie, Hudson, NH, USA) with a Biorobotics Microgrid II microarray printer (Genomic Solutions). Microspot 10K quill pins (Biorobotics) in a 48-pin tool were used to deposit <0.5 nL per spot onto the slide. The resulting microarrays have a 4 x12 subgrid layout with 392 spots per subgrid, each spot having approximate diameter and pitch of 90 µm and 190µm, respectively. A 280-bp GFP (green fluorescent protein) oligo was printed in subgrid corners to assist in placing on the grid. The slides were crosslinked in a UV Stratalinker 2400 (Stratagene) at 300mJ. Array quality was assessed by visual inspection and hybridization of representative slides from a print run by dye-labelled random 9-mer oligonucleotides. The quality control images were aquired via the ScanArray Express from Perkin Elmer at a 10 micron resolution and quantified with the Imagene 6.0 software suite.
Support
glass
Coating
aminosilane
Contributor(s)
Dangerfield B , Brahmbhatt S , Lehman M , Anderson S Submission date
Jun 14, 2006
Last update date
Jan 18, 2013
Contact name
Shawn Anderson
E-mail(s)
shawn.anderson@vch.ca Phone
604-875-4111 (63436)
Fax
604-875-5654
Organization name
University of British Columbia
Department
Urological Sciences
Lab
Microarray Facility, Dr. Colleen Nelson
Street address
The Prostate Centre at Vancouver General Hospital, 2660 Oak Street
City
Vancouver
State/province
B.C.
ZIP/Postal code
V6H 3Z6
Country
Canada
Samples (238)
GSM115459 , GSM115460 , GSM115461 , GSM115462 , GSM115463 , GSM115464
GSM115465 ,
GSM115466 ,
GSM115467 ,
GSM115468 ,
GSM115469 ,
GSM115470 ,
GSM115471 ,
GSM115472 ,
GSM115473 ,
GSM115474 ,
GSM115475 ,
GSM115476 ,
GSM115477 ,
GSM115478 ,
GSM115479 ,
GSM115480 ,
GSM115481 ,
GSM115482 ,
GSM115483 ,
GSM115484 ,
GSM115485 ,
GSM115486 ,
GSM115487 ,
GSM115488 ,
GSM115489 ,
GSM115490 ,
GSM115491 ,
GSM115492 ,
GSM115493 ,
GSM115494 ,
GSM115495 ,
GSM115496 ,
GSM115497 ,
GSM115498 ,
GSM115499 ,
GSM115500 ,
GSM115501 ,
GSM115502 ,
GSM115503 ,
GSM115504 ,
GSM115505 ,
GSM115506 ,
GSM115507 ,
GSM115508 ,
GSM115509 ,
GSM135971 ,
GSM135972 ,
GSM135973 ,
GSM135974 ,
GSM135975 ,
GSM135976 ,
GSM135977 ,
GSM135978 ,
GSM135983 ,
GSM135984 ,
GSM135985 ,
GSM135986 ,
GSM135987 ,
GSM135988 ,
GSM135990 ,
GSM135991 ,
GSM135992 ,
GSM135993 ,
GSM135994 ,
GSM135995 ,
GSM135996 ,
GSM135997 ,
GSM135998 ,
GSM135999 ,
GSM136000 ,
GSM136001 ,
GSM136002 ,
GSM136003 ,
GSM136004 ,
GSM136005 ,
GSM136006 ,
GSM136007 ,
GSM136008 ,
GSM136009 ,
GSM136010 ,
GSM136011 ,
GSM136012 ,
GSM136013 ,
GSM136014 ,
GSM136015 ,
GSM136016 ,
GSM136017 ,
GSM136018 ,
GSM136019 ,
GSM136020 ,
GSM136021 ,
GSM136022 ,
GSM136023 ,
GSM136024 ,
GSM136025 ,
GSM136026 ,
GSM136027 ,
GSM136028 ,
GSM136029 ,
GSM136030 ,
GSM136031 ,
GSM147100 ,
GSM147347 ,
GSM147348 ,
GSM147349 ,
GSM147351 ,
GSM147352 ,
GSM147353 ,
GSM147355 ,
GSM147383 ,
GSM147384 ,
GSM147385 ,
GSM147386 ,
GSM147387 ,
GSM147388 ,
GSM147389 ,
GSM147390 ,
GSM147527 ,
GSM147529 ,
GSM147532 ,
GSM147537 ,
GSM147541 ,
GSM147545 ,
GSM147548 ,
GSM147552 ,
GSM147553 ,
GSM147555 ,
GSM147658 ,
GSM147659 ,
GSM147660 ,
GSM147661 ,
GSM147662 ,
GSM206100 ,
GSM206166 ,
GSM206167 ,
GSM206168 ,
GSM206169 ,
GSM206170 ,
GSM206357 ,
GSM206358 ,
GSM206359 ,
GSM206360 ,
GSM206361 ,
GSM206362 ,
GSM300461 ,
GSM300734 ,
GSM300735 ,
GSM300736 ,
GSM300737 ,
GSM300738 ,
GSM300739 ,
GSM300740 ,
GSM300741 ,
GSM300742 ,
GSM300743 ,
GSM300744 ,
GSM300745 ,
GSM300746 ,
GSM300747 ,
GSM300748 ,
GSM315243 ,
GSM315245 ,
GSM315246 ,
GSM315247 ,
GSM315248 ,
GSM315250 ,
GSM315251 ,
GSM442624 ,
GSM442993 ,
GSM443033 ,
GSM443034 ,
GSM443035 ,
GSM443036 ,
GSM443037 ,
GSM443038 ,
GSM443039 ,
GSM443040 ,
GSM443041 ,
GSM443042 ,
GSM443043 ,
GSM443044 ,
GSM443045 ,
GSM443046 ,
GSM443047 ,
GSM443048 ,
GSM443049 ,
GSM443050 ,
GSM443051 ,
GSM443052 ,
GSM443053 ,
GSM443054 ,
GSM443055 ,
GSM443056 ,
GSM443057 ,
GSM443058 ,
GSM443059 ,
GSM443060 ,
GSM529604 ,
GSM529605 ,
GSM529606 ,
GSM529607 ,
GSM529608 ,
GSM529609 ,
GSM529610 ,
GSM529611 ,
GSM529612 ,
GSM529613 ,
GSM529614 ,
GSM529615 ,
GSM529616 ,
GSM529617 ,
GSM591265 ,
GSM591267 ,
GSM591268 ,
GSM591269 ,
GSM591271 ,
GSM591272 ,
GSM591273 ,
GSM591274 ,
GSM591275 ,
GSM591276 ,
GSM591277 ,
GSM591278 ,
GSM591279 ,
GSM591280 ,
GSM591281 ,
GSM591282 ,
GSM591283 ,
GSM591284 ,
GSM591286 ,
GSM591287 ,
GSM591289 Series (10)
GSE5123 Expression profiling defines a recurrence signature in lung squamous cell carcinoma
GSE5828 Gene expression profiling classifies lung squamous cell carcinomas with N1 positive lymph nodes
GSE5843 Expression profiling defines a recurrence signature in lung adenocarcinoma
GSE6520 Microarray analysis shows multiple signaling pathways are involved in basal cell carcinoma growth
GSE8325 Cytotoxic activity of HTI-286 in prostate cancer
GSE11905 Lichen Planopilaris and Pseudopelade of Brocq Involve Distinct Disease Associated Gene Expression Patterns by Microarray
GSE12542 Gene expression pattern similarity and differences between hair follicles and basal cell carcinomas
GSE17770 Expression Profiling Identifies Genes Involved in Emphysema
GSE20875 ADAM-28: A potential oncogene involved in asbestos-related lung adenocarcinomas
GSE24009 Deficiency in nucleotide excision repair (NER) family gene activity, especially ERCC3, is associated with non-pigmented hair fiber growth
Data table header descriptions ID Meta Row Field row
Meta Column Field column
Row Subgrid row
Column Subgrid column
Description Oligo probe description
Gene Symbol Official gene symbol
GB_LIST Refseq accession
Description Refseq Refseq description
GB_LIST Genbank accession
CLONE ID Gene ID
SPOT_ID
Data table ID Meta Row Meta Column Row Column Description Gene Symbol GB_LIST Description Refseq GB_LIST CLONE ID SPOT_ID 1.1.1.1
1
1
1
1
GFP Oligo Marker
-- CONTROL U17997
1.1.1.2
1
1
1
2
AK057513 H200020668
1.1.1.3
1
1
1
3
Q96M64
AK057355 H200020692
1.1.1.4
1
1
1
4
beta(1,6)-N-acetylglucosaminyltransferase V isoform 1; N-acetylglucosaminyltransferase IX; beta(1,6)-N-acetylglucosaminyltransferase V [Homo sapiens]. [Source:RefSeq;Acc:NM_144677]
MGAT5B
NM_198955,NM_144677 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isoenzyme B
AB114297 H200020626
1.1.1.5
1
1
1
5
AK057638 H200020650
1.1.1.6
1
1
1
6
AK057458 H200020674
1.1.1.7
1
1
1
7
AK057320 H200020698
1.1.1.8
1
1
1
8
vav-1 interacting Kruppel-like protein isoform b [Homo sapiens]. [Source:RefSeq;Acc:NM_024061]
NP_076966
NM_138494 zinc finger protein 655
BC011816,AK128057 H200021000
1.1.1.9
1
1
1
9
Transforming protein p21 (K-Ras 2) (Ki-Ras) (c-K-ras). [Source:Uniprot/SWISSPROT;Acc:P01116]
KRAS2
NM_033360,NM_004985 v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
BC010502 H200021024
1.1.1.10
1
1
1
10
AK056070 H200021048
1.1.1.11
1
1
1
11
AK056250 H200021072
1.1.1.12
1
1
1
12
AK054757 H200021006
1.1.1.13
1
1
1
13
M-phase phosphoprotein 9 (Fragment). [Source:Uniprot/SWISSPROT;Acc:Q99550]
MPHOSPH9
NM_022782 M-phase phosphoprotein 9
AK096541 H200021030
1.1.1.14
1
1
1
14
AK128256,AK057161 H200021054
1.1.1.15
1
1
1
15
pseudoautosomal GTP-binding protein-like protein; pseudoautosomal GTP-binding protein-like [Homo sapiens]. [Source:RefSeq;Acc:NM_012227]
GTPBP6
NM_012227 GTP binding protein 6 (putative)
Y14391,AK097872 H200021078
1.1.1.16
1
1
1
16
3xSSC
-- 3xSSC
1.1.1.17
1
1
1
17
3xSSC
-- 3xSSC
1.1.1.18
1
1
1
18
3xSSC
-- 3xSSC
1.1.1.19
1
1
1
19
3xSSC
-- 3xSSC
1.1.1.20
1
1
1
20
3xSSC
-- 3xSSC
23232 3224 Kbytes .Supplementary data files not provided
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