GEO DataSets

(Submitter supplied) Our group has developed an extra-uterine environment for newborn development (EXTEND) using an ovine model, that aims to mimic the womb to improve short and long-term health outcomes associated with prematurity. This study’s objective was to determine the histologic and transcriptomic consequences of EXTEND on the brain. Histology and RNA-sequencing was conducted on brain tissue from three cohorts of lambs: control pre-term (106-107 days), control late pre-term (127 days), and experimental lambs who were born pre-term and supported on EXTEND until late pre-term age (125-128 days). more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15670

36 Samples

Download data: CSV

(Submitter supplied) the hypothalamus tissues of high-reproduction small-tailed Han sheep and low-reproduction Wadi sheep were collected, and full-length transcriptome sequencing by Oxford Nanopore Technologies (ONT) was performed to explore the key functional genes associated with sheep fecundity. The differentially expressed genes (DEGs) were screened and enriched using DESeq2 software through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).

Organism:

Ovis aries

Type:

Other

Platform:

GPL34350

6 Samples

Download data: XLS

(Submitter supplied) Feed restriction during the early life of ewe lambs (F0) triggers the transfer of epigenetic marks to the next generation, thus impairing the reproductive performance of F1. However, the effects of this factor on milk production and composition, including its abundance in regulatory miRNA (many of which are contained in exosomes, small vesicles of endocytic origin that play a role in the modulation of immune response of the offspring), has not been tested so far. more...

Organism:

Ovis aries

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL31284

8 Samples

Download data: TXT

(Submitter supplied) We report the side-effects of ZEA on the testis,

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

2 Samples

Download data: MTX, TSV

(Submitter supplied) In the current study, five late gestation multiparous ewes were restricted to a 30% feeding level to create a model of malnutrition, while five other ewes were fed normally as controls. All ewes were sacrificed and jejunal and ileal samples were collected for transcriptome sequencing to study metabolic changes.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19778

20 Samples

Download data: XLSX

(Submitter supplied) The preservation of locally endangered breeds is essential for ecosystem services that affect both society and the environment. Reproductive fitness becomes a crucial consideration in this regard. MicroRNAs (miRNAs) are small noncoding RNA molecules with a key role in post-transcriptional regulation. They usually act in tissues, where they are produced, but when released in extracellular fluid, they are termed circulating miRNAs (c-miRNAs). more...

Organism:

Ovis aries

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL27721

12 Samples

Download data: TXT, XLSX

(Submitter supplied) This study identifies key microbiome and epithelial cell subtypes involved in grass digestion and VFA metabolism in the rumen. By integrating multi-omic data, we reveal novel links between microbial activity, epithelial cell function, and grassland foraging, providing critical insights into mechanisms underlying grass prevalence and their implications for optimizing ruminant health and productivity. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

6 Samples

Download data: MTX, TSV

(Submitter supplied) The methylation landscape of the sheep Y-chromosome was characterized using methylated cytosine data produced from PacBio and ONT long reads sequencing platforms. The study aimed to corroborate the presumptive locus of the sheep Y-chromosome centromere.

Organism:

Ovis aries

Type:

Methylation profiling by high throughput sequencing

Platforms:

GPL34349 GPL34350

2 Samples

Download data: BED

(Submitter supplied) The fat tail of sheep is an important organ that has evolved to adapt to extreme environmental conditions. Mesenchyme homeobox 2 (MEOX2) can inhibit adipogenic differentiation of stromal vascular fractions (SVFs) isolated from ovine tail adipose tissue. However, the underlying mechanism through which MEOX2 regulates ovine adipogenesis remains unclear. Therefore, this study aims to employ RNA sequencing (RNA-seq) to explore the downstream genes regulated by MEOX2 during the adipogenic differentiation of ovine SVFs. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

6 Samples

Download data: TXT

(Submitter supplied) [Background] Antenatal steroid (ACS) therapy conveys lifesaving benefits to the preterm fetus. However, current treatments are associated with increased risk of growth, endocrine, and neurodevelopmental abnormalities. [Objectives] To inform ACS therapy optimisation we used a sheep model of pregnancy to test whether adverse fetal brain effects were independent of respiratory benefit and ACS pharmacokinetics. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

48 Samples

Download data: TSV

(Submitter supplied) Aluminum hydroxide, has long been employed as a vaccine adjuvant for its safety profile, although its precise mechanism of action remains elusive. In this study, we investigated the transcriptomic responses in sheep spleen following repetitive vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. Notably, this work represents the first exploration of the sheep spleen transcriptome in such conditions. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

18 Samples

Download data: TXT

(Submitter supplied) Improving the fertility of sheep is an important goal in sheep breeding as it greatly increases the productivity. Dolang sheep is a typical representative breed of lamb in Xinjiang and is the main local sheep breed and meat source in the region. To explore the genes associated with the initiation of puberty in Dolang sheep, the hypothalamic tissues of Dolang sheep prepubertal, pubertal, and postpubertal periods were collected for RNA-seq analysis on the Illumina platform.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15670

9 Samples

Download data: TXT

(Submitter supplied) Feeding dams during gestation affects the development of the offspring for their entire life. The objective of the current experiment was to evaluate the changes of the transcriptome in the hypothalamus of the offspring lambs born from dams supplemented with: i) a control diet (without lipids or methionine supplementation), ii) an omega 3 fatty acid supplementation, or iii) a methionine supplementation. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30257

17 Samples

Download data: SF

(Submitter supplied) This study aimed to evaluate the effect of including soybean molasses (SM) on performance, blood parameters, carcass traits, meat quality, fatty acid and muscle (longissimus thoracis) transcriptomic profiles of castrated lambs. Twenty Dorper × Santa Inês lambs (20.06 ± 0.76 kg BW) were assigned to a randomized block design, stratified by BW, with the following treatments: CON - 0 g/kg of SM and SM20 - 200 g/kg of SM on DM basis, allocated in individual pens. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19778

8 Samples

Download data: CSV, XLSX

(Submitter supplied) Zygotic genome activation (ZGA) is essential for early embryo development. Here, we reported that ZGA is initiated at 16-cell stage in sheep, as extensive alterations in gene expression and DNA methylation patterns, along with elevated levels of RNA polymerase II and developmental arrest was found at the 16-cell embryos. To uncover the sophisticated RNA metabolism during ZGA, we conducted weighted gene co-expression network analysis and identified 1957 critical maternal genes. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23810

26 Samples

Download data: TXT

(Submitter supplied) In the Ross procedure, a patient's pulmonary valve is transplanted into aortic position. Reoperation is needed in many cases due to failure through dilatation of the pulmonary autograft. To further understand the failure mechanisms, gene expression profiling of the autograft tissue was performed and compared to control aortic and pulmonary sinus tissue though bulk RNA-seq. Notable results include the upregulation of inflammatory pathways and genes coding for proteins important for the structural integrity of the tissue, such as extracellular matrix proteins, collagenases and elastases.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

22 Samples

Download data: TXT

(Submitter supplied) Background: In mammals, female fertility is determined by the outcome of follicular development (ovulation or atresia). Follicular atresia is a complex physiological process that results in the degeneration of oocytes from the ovary. However, the molecular mechanisms of oocyte degeneration and key protein markers of follicular atresia remain unclear. In this study, we explored the complex transcriptional regulatory mechanisms and protein profiles in oocytes and follicular fluid in atretic follicle stages using single-cell RNA sequencing and tandem mass tag proteomics. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

6 Samples

Download data: TXT

(Submitter supplied) We perform transcriptomic sequencing of rumen, reticulum, omasum, and abomasum epithelial tissues from developing lambs. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points (day5 and day10).

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

24 Samples

Download data: CSV

(Submitter supplied) We perform transcriptomic sequencing of rumen, reticulum, omasum, and abomasum epithelial tissues from developing lambs.

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

24 Samples

Download data: CSV

(Submitter supplied) Glucose, the primary energy substrate for fetal oxidative processes and growth, is transferred from maternal to fetal circulation down a concentration gradient by placental facili-tative glucose transporters. In sheep, SLC2A1 and SLC2A3 are the primary transporters available in the placental epithelium, with SLC2A3 located on the maternal-facing apical trophoblast membrane and SLC2A1 located on the fetal-facing basolateral trophoblast membrane. more...

Organism:

Ovis aries

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27721

8 Samples

Download data: XLSX