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IFI16 interferon gamma inducible protein 16 [ Homo sapiens (human) ]

Gene ID: 3428, updated on 14-Nov-2024

Summary

Official Symbol
IFI16provided by HGNC
Official Full Name
interferon gamma inducible protein 16provided by HGNC
Primary source
HGNC:HGNC:5395
See related
Ensembl:ENSG00000163565 MIM:147586; AllianceGenome:HGNC:5395
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
PYHIN2; IFNGIP1
Summary
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
Expression
Ubiquitous expression in lymph node (RPKM 45.3), appendix (RPKM 41.3) and 24 other tissues See more
Orthologs
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Genomic context

See IFI16 in Genome Data Viewer
Location:
1q23.1
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (158999976..159055151)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (158137044..158192189)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (158969766..159024941)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:158856217-158856716 Neighboring gene pyrin and HIN domain family member 5, pseudogene Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:158879152-158879652 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:158908087-158909286 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:158922455-158923423 Neighboring gene pyrin and HIN domain family member 1 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:158928812-158928990 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1900 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:158975177-158975702 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:158975703-158976226 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1901 Neighboring gene uncharacterized LOC124904432 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1452 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:158984807-158986006 Neighboring gene pyrin domain containing 5 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1902 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1903 Neighboring gene absent in melanoma 2 Neighboring gene RAD1 pseudogene 2 Neighboring gene tRNA-Arg (anticodon TCT) 4-1

Genomic regions, transcripts, and products

Expression

  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phenotypes

EBI GWAS Catalog

Description
Genome-wide association and population genetic analysis of C-reactive protein in African American and Hispanic American women.
EBI GWAS Catalog
GWAS of blood cell traits identifies novel associated loci and epistatic interactions in Caucasian and African-American children.
EBI GWAS Catalog
Meta-analysis of genome-wide association studies in celiac disease and rheumatoid arthritis identifies fourteen non-HLA shared loci.
EBI GWAS Catalog
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
EBI GWAS Catalog

HIV-1 interactions

Protein interactions

Protein Gene Interaction Pubs
Nef nef Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Nef may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed
Pr55(Gag) gag HIV-1 Gag interacts with IfI16 as demonstrated by proximity dependent biotinylation proteomics PubMed
Tat tat Microarray analysis indicates HIV-1 Tat upregulates the interferon-responsive gene expression of many proteins, including IFI16, in immature dendritic cells, an effect that likely facilitates the expansion of HIV-1 infection PubMed
Vpr vpr Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Vpr may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed
Vpu vpu Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Vpu may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Interactions

Products Interactant Other Gene Complex Source Pubs Description

General gene information

Markers

Clone Names

  • MGC9466

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA binding HDA PubMed 
enables double-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
 
enables double-stranded DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
enables transcription factor binding IDA
Inferred from Direct Assay
more info
PubMed 
Process Evidence Code Pubs
involved_in activation of cysteine-type endopeptidase activity IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in activation of innate immune response IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in activation of innate immune response IDA
Inferred from Direct Assay
more info
PubMed 
involved_in autophagy IEA
Inferred from Electronic Annotation
more info
 
involved_in cellular response to glucose starvation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in cellular response to interferon-beta IBA
Inferred from Biological aspect of Ancestor
more info
 
involved_in cellular response to ionizing radiation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in defense response to virus IDA
Inferred from Direct Assay
more info
PubMed 
involved_in defense response to virus IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in inflammatory response IEA
Inferred from Electronic Annotation
more info
 
involved_in innate immune response IEA
Inferred from Electronic Annotation
more info
 
involved_in intrinsic apoptotic signaling pathway by p53 class mediator IMP
Inferred from Mutant Phenotype
more info
PubMed 
acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator IDA
Inferred from Direct Assay
more info
PubMed 
involved_in monocyte differentiation IDA
Inferred from Direct Assay
more info
PubMed 
involved_in myeloid cell differentiation NAS
Non-traceable Author Statement
more info
PubMed 
involved_in negative regulation of AIM2 inflammasome complex assembly IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of cysteine-type endopeptidase activity IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of gene expression, epigenetic IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of innate immune response IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
involved_in negative regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in negative regulation of viral genome replication IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of cytokine production TAS
Traceable Author Statement
more info
PubMed 
involved_in positive regulation of interleukin-1 beta production IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
PubMed 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
PubMed 
involved_in regulation of autophagy IEP
Inferred from Expression Pattern
more info
PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 
colocalizes_with cytosol IPI
Inferred from Physical Interaction
more info
PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
 
located_in membrane HDA PubMed 
located_in nuclear speck IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in nucleolus IDA
Inferred from Direct Assay
more info
PubMed 
is_active_in nucleoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
PubMed 

General protein information

Preferred Names
gamma-interferon-inducible protein 16
Names
interferon-gamma induced protein IFI 16
interferon-inducible myeloid differentiation transcriptional activator

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_029141.2 RefSeqGene

    Range
    14938..60182
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001206567.2NP_001193496.1  gamma-interferon-inducible protein 16 isoform 1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) lacks an in-frame exon in the 5' coding region and has an additional in-frame exon in the 3' coding region, compared to variant 2. The additional exon is described as exon 7a in PMID:9718316. The resulting isoform (1) is of the same size but lacks a segment in the N-terminal region and has an additional segment in the C-terminal region, compared to isoform 2. Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.
    Source sequence(s)
    AK296228, BM806453, DA945454, DB273519, M63838
    Consensus CDS
    CCDS58039.1
    Related
    ENSP00000352740.3, ENST00000359709.7
    Conserved Domains (3) summary
    cd08305
    Location:1083
    Pyrin; Pyrin: a protein-protein interaction domain
    pfam02760
    Location:145313
    HIN; HIN-200/IF120x domain
    pfam06583
    Location:343545
    Neogenin_C; Neogenin C-terminus
  2. NM_001364867.2NP_001351796.1  gamma-interferon-inducible protein 16 isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) encodes the longest isoform (3, also known as IFI 16A).
    Source sequence(s)
    AL359753
    Consensus CDS
    CCDS91079.1
    UniProtKB/Swiss-Prot
    A0A346JEQ3, B4DJT8, H3BLV7, Q16666, Q59GX0, Q5T3W7, Q5T3W8, Q5T3X0, Q5T3X1, Q5T3X2, Q8N9E5, Q8NEQ7, Q96AJ5, Q9UH78
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain
  3. NM_001376587.1NP_001363516.1  gamma-interferon-inducible protein 16 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Consensus CDS
    CCDS91079.1
    UniProtKB/Swiss-Prot
    A0A346JEQ3, B4DJT8, H3BLV7, Q16666, Q59GX0, Q5T3W7, Q5T3W8, Q5T3X0, Q5T3X1, Q5T3X2, Q8N9E5, Q8NEQ7, Q96AJ5, Q9UH78
    Related
    ENSP00000295809.7, ENST00000295809.12
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain
  4. NM_001376588.1NP_001363517.1  gamma-interferon-inducible protein 16 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Consensus CDS
    CCDS1180.3
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain
  5. NM_001376589.1NP_001363518.1  gamma-interferon-inducible protein 16 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Consensus CDS
    CCDS1180.3
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain
  6. NM_001376591.1NP_001363520.1  gamma-interferon-inducible protein 16 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Consensus CDS
    CCDS91080.1
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain
  7. NM_001376592.1NP_001363521.1  gamma-interferon-inducible protein 16 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AL359753
    Consensus CDS
    CCDS91080.1
    Related
    ENSP00000404325.2, ENST00000448393.6
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain
  8. NM_005531.3NP_005522.2  gamma-interferon-inducible protein 16 isoform 2

    See identical proteins and their annotated locations for NP_005522.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) consists of 11 exons and encodes isoform 2 (also known as isoform B, PMIDs:9718316 and 12588705). Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.
    Source sequence(s)
    AL359753, BC017059, DA990524, M63838
    Consensus CDS
    CCDS1180.3
    Related
    ENSP00000357113.4, ENST00000368131.8
    Conserved Domains (3) summary
    PTZ00449
    Location:100215
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    cd08305
    Location:1083
    Pyrin; a protein-protein interaction domain
    pfam02760
    Location:201369
    HIN; HIN-200/IF120x domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    158999976..159055151
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047419411.1XP_047275367.1  gamma-interferon-inducible protein 16 isoform X2

  2. XM_047419406.1XP_047275362.1  gamma-interferon-inducible protein 16 isoform X1

  3. XM_047419399.1XP_047275355.1  gamma-interferon-inducible protein 16 isoform X1

  4. XM_047419413.1XP_047275369.1  gamma-interferon-inducible protein 16 isoform X2

RNA

  1. XR_007059344.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    158137044..158192189
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054336307.1XP_054192282.1  gamma-interferon-inducible protein 16 isoform X1

  2. XM_054336310.1XP_054192285.1  gamma-interferon-inducible protein 16 isoform X2

  3. XM_054336308.1XP_054192283.1  gamma-interferon-inducible protein 16 isoform X1

  4. XM_054336306.1XP_054192281.1  gamma-interferon-inducible protein 16 isoform X1

  5. XM_054336309.1XP_054192284.1  gamma-interferon-inducible protein 16 isoform X2

RNA

  1. XR_008486021.1 RNA Sequence

  2. XR_008486020.1 RNA Sequence