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NONO non-POU domain containing octamer binding [ Homo sapiens (human) ]

Gene ID: 4841, updated on 10-Dec-2024

Summary

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Official Symbol
NONOprovided by HGNC
Official Full Name
non-POU domain containing octamer bindingprovided by HGNC
Primary source
HGNC:HGNC:7871
See related
Ensembl:ENSG00000147140 MIM:300084; AllianceGenome:HGNC:7871
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
Summary
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
Expression
Ubiquitous expression in ovary (RPKM 81.7), lymph node (RPKM 64.3) and 25 other tissues See more
Orthologs
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Genomic context

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See NONO in Genome Data Viewer
Location:
Xq13.1
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) X NC_000023.11 (71283635..71301168)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) X NC_060947.1 (69717744..69735278)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (70503485..70521018)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene gap junction protein beta 1 Neighboring gene zinc finger MYM-type containing 3 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20895 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20896 Neighboring gene uncharacterized LOC105373247 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chrX:70523398-70524597 Neighboring gene integrin subunit beta 1 binding protein 2 Neighboring gene ZCRB1 pseudogene 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Tissue-specific circular RNA induction during human fetal development
  • Description: 35 human fetal samples from 6 tissues (3 - 7 replicates per tissue) collected between 10 and 20 weeks gestational time were sequenced using Illumina TruSeq Stranded Total RNA
  • BioProject: PRJNA270632
  • Publication: PMID 26076956
  • Analysis date: Mon Apr 2 22:54:59 2018

Bibliography

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Related articles in PubMed

  1. p54nrb is a new regulator of progression of malignant melanoma. Schiffner S, et al. Carcinogenesis, 2011 Aug. PMID 21642354
  2. 55 kDa nuclear matrix protein (nmt55) mRNA is expressed in human prostate cancer tissue and is associated with the androgen receptor. Ishiguro H, et al. Int J Cancer, 2003 May 20. PMID 12672026
  3. p54(nrb) associates with the 5' splice site within large transcription/splicing complexes. Kameoka S, et al. EMBO J, 2004 Apr 21. PMID 15057275, Free PMC Article
  4. Genetic and phenotypic spectrum in the NONO-associated syndromic disorder. Roessler F, et al. Am J Med Genet A, 2023 Feb. PMID 36426740
  5. The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions. Eichler M, et al. Cell Death Dis, 2022 Apr 20. PMID 35444189, Free PMC Article

See all (418) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Nucleolar detention of NONO shields DNA double-strand breaks from aberrant transcripts.
    Title: Nucleolar detention of NONO shields DNA double-strand breaks from aberrant transcripts.
  2. NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.
    Title: NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.
  3. IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.
    Title: IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.
  4. The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.
    Title: The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA.
  5. Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/beta-catenin pathways in human breast cancer cells.
    Title: Non-POU Domain-Containing Octomer-Binding (NONO) protein expression and stability promotes the tumorigenicity and activation of Akt/MAPK/β-catenin pathways in human breast cancer cells.
  6. NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.
    Title: NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.
  7. A novel NONO variant that causes developmental delay and cardiac phenotypes.
    Title: A novel NONO variant that causes developmental delay and cardiac phenotypes.
  8. NONO regulates multiple cytokine production in sepsis via the ERK1/2 signaling pathway.
    Title: NONO regulates multiple cytokine production in sepsis via the ERK1/2 signaling pathway.
  9. Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.
    Title: Targeting the splicing factor NONO inhibits GBM progression through GPX1 intron retention.
  10. Molecular Modelling of NONO and SFPQ Dimerization Process and RNA Recognition Mechanism.
    Title: Molecular Modelling of NONO and SFPQ Dimerization Process and RNA Recognition Mechanism.
Submit: New GeneRIF Correction See all GeneRIFs (92)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description Tests
Syndromic X-linked intellectual disability 34
MedGen: C4225417 OMIM: 300967 GeneReviews: Not available
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Copy number response

Description
Copy number response
Triplosensitivity

No evidence available (Last evaluated 2024-07-24)

ClinGen Genome Curation Page
Haploinsufficency

Sufficient evidence for dosage pathogenicity (Last evaluated 2024-07-24)

ClinGen Genome Curation PagePubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of NONO by shRNA enhances HIV-1 replication in both Jurkat cells and primary CD4+ T-cells PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells PubMed
gag Tandem affinity purification and mass spectrometry analysis identify non-POU domain containing octamer-binding protein (NONO), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Rev rev Co-immunoprecipitation analysis demonstrates that endogenous RBM14 associates with HIV-1 Rev, XPO1, and p54/NRB in cells PubMed
rev HIV-1 Rev interacting protein, non-POU domain containing, octamer-binding (NONO), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with NONO is increased by RRE PubMed
Vpr vpr A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of non-POU domain containing octamer-binding protein (NONO) expression by HIV-1 Vpr in Vpr transduced macrophages PubMed
capsid gag Knockdown of NONO expression by shRNA enhances both HIV-1 Gag expression in Jurkat cells and the released p24 levels from these cells PubMed
retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human non-POU domain containing, octamer-binding (NONO) at amino acid residues 54-55, 274-275, and 277-278 by the HIV-1 protease PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA binding HDA PubMed 
enables RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables chromatin binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in activation of innate immune response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in innate immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of DNA-templated transcription ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of circadian rhythm ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
part_of RNA polymerase II transcription regulator complex ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in fibrillar center IDA
Inferred from Direct Assay
more info
  
located_in membrane HDA PubMed 
located_in nuclear matrix IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear speck IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in paraspeckles IDA
Inferred from Direct Assay
more info
 PubMed 
located_in paraspeckles IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
non-POU domain-containing octamer-binding protein
Names
54 kDa nuclear RNA- and DNA-binding protein
55 kDa nuclear protein
DNA-binding p52/p100 complex, 52 kDa subunit
non-POU domain-containing octamer (ATGCAAAT) binding protein
p54(nrb)
protein phosphatase 1, regulatory subunit 114

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_046742.1 RefSeqGene

    Range
    5444..22977
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001145408.2 → NP_001138880.1  non-POU domain-containing octamer-binding protein isoform 1

    See identical proteins and their annotated locations for NP_001138880.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
    Source sequence(s)
    BC002364, BC003129, DA116840
    Consensus CDS
    CCDS14410.1
    UniProtKB/Swiss-Prot
    B7Z4C2, D3DVV4, F5GYZ3, O00201, P30807, Q12786, Q15233, Q9BQC5
    UniProtKB/TrEMBL
    A0A0S2Z4Z9, A8K525
    Related
    ENSP00000410299.2, ENST00000420903.6
    Conserved Domains (3) summary
    cd12946
    Location:219 → 311
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cd12588
    Location:73 → 143
    RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
    cl17169
    Location:149 → 228
    RRM_SF; RNA recognition motif (RRM) superfamily

  2. NM_001145409.2 → NP_001138881.1  non-POU domain-containing octamer-binding protein isoform 1

    See identical proteins and their annotated locations for NP_001138881.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).
    Source sequence(s)
    BC002364, BC012141, DA116840
    Consensus CDS
    CCDS14410.1
    UniProtKB/Swiss-Prot
    B7Z4C2, D3DVV4, F5GYZ3, O00201, P30807, Q12786, Q15233, Q9BQC5
    UniProtKB/TrEMBL
    A0A0S2Z4Z9, A8K525
    Related
    ENSP00000362947.1, ENST00000373841.5
    Conserved Domains (3) summary
    cd12946
    Location:219 → 311
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cd12588
    Location:73 → 143
    RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
    cl17169
    Location:149 → 228
    RRM_SF; RNA recognition motif (RRM) superfamily

  3. NM_001145410.2 → NP_001138882.1  non-POU domain-containing octamer-binding protein isoform 2

    See identical proteins and their annotated locations for NP_001138882.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AK297144, BC002364
    Consensus CDS
    CCDS55445.1
    UniProtKB/TrEMBL
    A8K525
    Related
    ENSP00000441364.1, ENST00000535149.5
    Conserved Domains (2) summary
    cd12946
    Location:130 → 222
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cl17169
    Location:60 → 139
    RRM_SF; RNA recognition motif (RRM) superfamily

  4. NM_007363.5 → NP_031389.3  non-POU domain-containing octamer-binding protein isoform 1

    See identical proteins and their annotated locations for NP_031389.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).
    Source sequence(s)
    BC002364, BC028299, DA116840
    Consensus CDS
    CCDS14410.1
    UniProtKB/Swiss-Prot
    B7Z4C2, D3DVV4, F5GYZ3, O00201, P30807, Q12786, Q15233, Q9BQC5
    UniProtKB/TrEMBL
    A0A0S2Z4Z9, A8K525
    Related
    ENSP00000276079.8, ENST00000276079.13
    Conserved Domains (3) summary
    cd12946
    Location:219 → 311
    NOPS_p54nrb_PSF_PSPC1; NOPS domain, including C-terminal coiled-coil region, in p54nrb/PSF/PSPC1 family proteins
    cd12588
    Location:73 → 143
    RRM1_p54nrb; RNA recognition motif 1 (RRM1) found in vertebrate 54 kDa nuclear RNA- and DNA-binding protein (p54nrb)
    cl17169
    Location:149 → 228
    RRM_SF; RNA recognition motif (RRM) superfamily

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p14 Primary Assembly

    Range
    71283635..71301168
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060947.1 Alternate T2T-CHM13v2.0

    Range
    69717744..69735278
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q15233.4 GenPept UniProtKB/Swiss-Prot:Q15233

Gene LinkOut

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