DMC1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DMC1
Primary source: SGD:S000000981
Locus tag: YER179W
See related: AllianceGenome:SGD:S000000981; FungiDB:YER179W; VEuPathDB:YER179W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ISC2
Summary: Enables ATPase inhibitor activity; DNA binding activity; and DNA strand exchange activity. Involved in meiotic joint molecule formation and synaptonemal complex assembly. Located in condensed nuclear chromosome. Orthologous to human DMC1 (DNA meiotic recombinase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: V

Exon count:: 2

Sequence:: Chromosome: V; NC_001137.3 (548421..549517)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Dmc1 recombinase physically interacts with and promotes the meiotic crossover functions of the Mlh1-Mlh3 endonuclease.
Title: The Dmc1 recombinase physically interacts with and promotes the meiotic crossover functions of the Mlh1-Mlh3 endonuclease.
The role of conserved amino acid residues of Sae3 in Mei5-Sae3 complex for Dmc1 assembly in meiotic recombination.
Title: The role of conserved amino acid residues of Sae3 in Mei5-Sae3 complex for Dmc1 assembly in meiotic recombination.
Rad51 facilitates filament assembly of meiosis-specific Dmc1 recombinase.
Title: Rad51 facilitates filament assembly of meiosis-specific Dmc1 recombinase.
Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms.
Title: Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms.
In spite of its ability to load onto single-stranded DNA and carry out recombination in the absence of Mei5-Sae3, recombination promoted by the Dmc1 mutant is abnormal in that it forms foci in the absence of DNA breaks, displays unusually high levels of multi-chromatid and intersister joint molecule intermediates, as well as high levels of ectopic recombination products.
Title: A mutant form of Dmc1 that bypasses the requirement for accessory protein Mei5-Sae3 reveals independent activities of Mei5-Sae3 and Rad51 in Dmc1 filament stability.
RPA is particularly important for the high level of Dmc1 activity in a reconstituted system, with three distinct mechanisms contributing to RPA's overall activity.
Title: RPA resolves conflicting activities of accessory proteins during reconstitution of Dmc1-mediated meiotic recombination.
propose that Rad51 and Dmc1 have intrinsic capacities to form spatially distinct filaments, suggesting that additional recombination cofactors are not required to segregate the Rad51 and Dmc1 filaments
Title: Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments.
We identified most known oxygen-regulated genes and also uncovered new regulated genes. Reverse transcription-quantitative PCR (RT-qPCR) analysis confirmed that the lysine methyltransferase EFM6 and the recombinase DMC1, both conserved in humans, are indeed oxygen-responsive.
Title: Time-Course Analysis of Gene Expression During the Saccharomyces cerevisiae Hypoxic Response.
models for how sequence imperfections may affect base triplet recognition by Rad51/RecA family members, and we discuss how these models and our results may relate to the different biological roles of RecA, Rad51, and Dmc1.
Title: Sequence imperfections and base triplet recognition by the Rad51/RecA family of recombinases.
Study provides evidence that both Hop2 and Sae3 promote homolog bias through a Dmc1-mediated double-strand break repair during meiosis.
Title: Hop2 and Sae3 Are Required for Dmc1-Mediated Double-Strand Break Repair via Homolog Bias during Meiosis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent DNA damage sensor activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IBA Inferred from Biological aspect of Ancestor more info
enables ATPase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA strand exchange activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA strand exchange activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA strand exchange activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombinase assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA strand invasion	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromosome organization involved in meiotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic joint molecule formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IEA Inferred from Electronic Annotation more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sporulation resulting in formation of a cellular spore	IEA Inferred from Electronic Annotation more info
involved_in synaptonemal complex assembly	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in condensed nuclear chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: recombinase DMC1

NP_011106.1

Meiosis-specific recombinase required for double-strand break repair; also required for pairing between homologous chromosomes and for the normal morphogenesis of synaptonemal complex; binds ssDNA and dsDNA, forms helical filaments; potent inhibitor of the ATPase activity of Srs2p helicase, blocking its ssDNA translocating motor activity and inhibiting its antirecombinase activity; stimulated by Rdh54p; homolog of Rad51p and the bacterial RecA protein