NOX5 NADPH oxidase 5 [Homo sapiens (human)] - Gene

Summary

Official Symbol: NOX5provided by HGNC
Official Full Name: NADPH oxidase 5provided by HGNC
Primary source: HGNC:HGNC:14874
See related: MIM:606572; AllianceGenome:HGNC:14874
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary: Enables proton channel activity and superoxide-generating NAD(P)H oxidase activity. Involved in several processes, including positive regulation of reactive oxygen species metabolic process; proton transmembrane transport; and superoxide anion generation. Located in endoplasmic reticulum and plasma membrane. [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in spleen (RPKM 7.9), testis (RPKM 4.2) and 3 other tissues See more
Orthologs: all
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Genomic context

Location:: 15q23

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	15	NC_000015.10 (69014695..69062762)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	15	NC_060939.1 (66837229..66885315)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	15	NC_000015.9 (69307034..69355102)

Chromosome 15 - NC_000015.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Role of NOX1 and NOX5 in protein kinase C/reactive oxygen species-mediated MMP-9 activation and invasion in MCF-7 breast cancer cells.
Title: Role of NOX1 and NOX5 in protein kinase C/reactive oxygen species‑mediated MMP‑9 activation and invasion in MCF‑7 breast cancer cells.
Structural basis of human NOX5 activation.
Title: Structural basis of human NOX5 activation.
Structure, regulation, and physiological functions of NADPH oxidase 5 (NOX5).
Title: Structure, regulation, and physiological functions of NADPH oxidase 5 (NOX5).
Ca[2+] -binding-region-dependent cell surface localization of NADPH oxidase Nox5.
Title: Ca(2+) -binding-region-dependent cell surface localization of NADPH oxidase Nox5.
NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients.
Title: NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients.
Impact of calcium and reactive oxygen species on human sperm function: Role of NOX5.
Title: Impact of calcium and reactive oxygen species on human sperm function: Role of NOX5.
Nicotine promotes vascular calcification via intracellular Ca2+-mediated, Nox5-induced oxidative stress, and extracellular vesicle release in vascular smooth muscle cells.
Title: Nicotine promotes vascular calcification via intracellular Ca2+-mediated, Nox5-induced oxidative stress, and extracellular vesicle release in vascular smooth muscle cells.
Independent of Renox, NOX5 Promotes Renal Inflammation and Fibrosis in Diabetes by Activating ROS-Sensitive Pathways.
Title: Independent of Renox, NOX5 Promotes Renal Inflammation and Fibrosis in Diabetes by Activating ROS-Sensitive Pathways.
NOX5 mediates the crosstalk between tumor cells and cancer-associated fibroblasts via regulating cytokine network.
Title: NOX5 mediates the crosstalk between tumor cells and cancer-associated fibroblasts via regulating cytokine network.
NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells.
Title: NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells.

Submit: New GeneRIF Correction See all GeneRIFs (88)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study evaluating lipoprotein-associated phospholipase A2 mass and activity at baseline and after rosuvastatin therapy. EBI GWAS Catalog EBI GWAS CatalogPubMed
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	HIV-1 gp120 binding to CXCR4 induces NADPH oxidase-mediated production of superoxide radicals in neurons, which is involved in the activation of neutral sphingomyelinase	PubMed
Envelope surface glycoprotein gp160, precursor	env	HIV-1 gp160 inhibits the cellular immune response and enhances oxidative stress mediated by NADPH oxidase	PubMed
Tat	tat	PDGF-BB inhibits NADPH oxidase-mediated reactive oxygen species production induced by HIV-1 Tat and morphine treatment in neuroblastoma cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

SHGC-84716 (e-PCR)
- UniSTS:182940

SHGC-143620 (e-PCR)
- UniSTS:171983

STS-H15004 (e-PCR)
- UniSTS:69876

Readthrough SPESP1-NOX5

Readthrough gene: SPESP1-NOX5, Included gene: SPESP1

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables NADP binding	NAS Non-traceable Author Statement more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	NAS Non-traceable Author Statement more info	PubMed
enables heme binding	NAS Non-traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables proton channel activity	IDA Inferred from Direct Assay more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	IBA Inferred from Biological aspect of Ancestor more info
NOT contributes_to superoxide-generating NAD(P)H oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables superoxide-generating NAD(P)H oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables superoxide-generating NADPH oxidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in angiogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in apoptotic process	NAS Non-traceable Author Statement more info	PubMed
involved_in cytoskeleton-dependent cytokinesis	NAS Non-traceable Author Statement more info	PubMed
involved_in defense response	IBA Inferred from Biological aspect of Ancestor more info
involved_in endothelial cell proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cytokine production	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of reactive oxygen species metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proton transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of fusion of sperm to egg plasma membrane	NAS Non-traceable Author Statement more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info
involved_in superoxide anion generation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	TAS Traceable Author Statement more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: NADPH oxidase 5

Names: NADPH oxidase, EF-hand calcium binding domain 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_030464.2 RefSeqGene

Range

89172..137239

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184779.2 → NP_001171708.1 NADPH oxidase 5 isoform 2

See identical proteins and their annotated locations for NP_001171708.1

Status: REVIEWED

Description

Transcript Variant: This variant (2, alternately referred to as NOX5 alpha and v1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AC027088, AF353088, AI091544, DA868876, DQ413001

Consensus CDS

CCDS53954.1

UniProtKB/Swiss-Prot

Q96PH1

UniProtKB/TrEMBL

A3QRJ0

Conserved Domains (4) summary

cd06186
Location:420 → 737

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd00051
Location:97 → 164

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam01794
Location:268 → 374

Ferric_reduct; Ferric reductase like transmembrane component

pfam13499
Location:98 → 162

EF-hand_7; EF-hand domain pair
NM_024505.4 → NP_078781.3 NADPH oxidase 5 isoform 1

See identical proteins and their annotated locations for NP_078781.3

Status: REVIEWED

Description

Transcript Variant: This variant (1, alternately referred to as NOX5 gamma and v3) encodes the longest isoform (1).

Source sequence(s)

AC027088, AF353089, AI091544, DA868876, DQ413001

Consensus CDS

CCDS32276.2

UniProtKB/Swiss-Prot

B2RBJ4, Q08AN2, Q08AN3, Q8TEQ1, Q8TER4, Q96PH1, Q96PH2, Q96PJ8, Q96PJ9, Q9H6E0, Q9HAM8

Conserved Domains (4) summary

cd06186
Location:448 → 765

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd00051
Location:97 → 192

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

pfam01794
Location:296 → 402

Ferric_reduct; Ferric reductase like transmembrane component

pfam13499
Location:98 → 190

EF-hand_7; EF-hand domain pair