PRCP prolylcarboxypeptidase [Homo sapiens (human)] - Gene

Summary

Official Symbol: PRCPprovided by HGNC
Official Full Name: prolylcarboxypeptidaseprovided by HGNC
Primary source: HGNC:HGNC:9344
See related: Ensembl:ENSG00000137509 MIM:176785; AllianceGenome:HGNC:9344
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: PCP; HUMPCP
Summary: This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
Expression: Ubiquitous expression in placenta (RPKM 42.6), urinary bladder (RPKM 37.6) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 11q14.1

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	11	NC_000011.10 (82822936..82901644, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	11	NC_060935.1 (82759396..82838151, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	11	NC_000011.9 (82533978..82612686, complement)

Chromosome 11 - NC_000011.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Haplotype-based association study between PRCP gene polymorphisms and essential hypertension in Hani minority group from a remote region of China.
Title: Haplotype-based association study between PRCP gene polymorphisms and essential hypertension in Hani minority group from a remote region of China.
Genetic association study of prolylcarboxypeptidase polymorphisms with susceptibility to essential hypertension in the Yi minority of China: A case-control study based on an isolated population.
Title: Genetic association study of prolylcarboxypeptidase polymorphisms with susceptibility to essential hypertension in the Yi minority of China: A case-control study based on an isolated population.
Fast oxidation of alpha-melanocyte-stimulating hormone and derived peptides under laboratory conditions causes irreproducible results-Insights from studies of prolylcarboxypeptidase in human cell types.
Title: Fast oxidation of α-melanocyte-stimulating hormone and derived peptides under laboratory conditions causes irreproducible results-Insights from studies of prolylcarboxypeptidase in human cell types.
Using multiple -omics approach, study validated key molecules, such as CAP1, SHC1 and PRCP, that might play a significant role in IgA nephropathy pathogenesis.
Title: Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis.
For the first time, PRCP is identified as an apelin-cleaving enzyme.
Title: Prolyl carboxypeptidase purified from human placenta: its characterization and identification as an apelin-cleaving enzyme.
The decrease in PRCP levels in the first 24 h after stroke onset is associated with stroke severity and an unfavourable short-term stroke outcome
Title: Prolyl carboxypeptidase activity decline correlates with severity and short-term outcome in acute ischemic stroke.
PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation.
Title: Prolylcarboxypeptidase independently activates plasma prekallikrein (fletcher factor).
The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population.
Title: Association of polymorphisms in prolylcarboxypeptidase and chymase genes with essential hypertension in the Chinese Han population.
PRCP regulates cell growth, angiogenesis, and the response to vascular injury
Title: Prolylcarboxypeptidase promotes angiogenesis and vascular repair.
analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors
Title: The discovery of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See Variation Viewer (GRCh37.p13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D11S1365 (e-PCR), detects polymorphism
- UniSTS:70659

SGC34782 (e-PCR)
- UniSTS:27401

RH92893 (e-PCR)
- UniSTS:91662

D11S2617 (e-PCR)
- UniSTS:51988

D11S4598 (e-PCR)
- UniSTS:12828

D11S1365 (e-PCR), detects polymorphism
- UniSTS:53025

STS-L13977 (e-PCR)
- UniSTS:82257

SHGC-36305 (e-PCR)
- UniSTS:21329

D11S4568 (e-PCR)
- UniSTS:67424

PRCP_1071 (e-PCR)
- UniSTS:277613

D1S1423 (e-PCR)
- UniSTS:149619

SHGC-132051 (e-PCR)
- UniSTS:170616

G62002 (e-PCR)
- UniSTS:139222

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables dipeptidyl-peptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serine-type carboxypeptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in angiogenesis involved in wound healing	IBA Inferred from Biological aspect of Ancestor more info
involved_in energy homeostasis	IEA Inferred from Electronic Annotation more info
involved_in glucose homeostasis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of systemic arterial blood pressure	IBA Inferred from Biological aspect of Ancestor more info
involved_in plasma kallikrein-kinin cascade	IEA Inferred from Electronic Annotation more info
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
involved_in regulation of blood vessel endothelial cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of reactive oxygen species metabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of thyroid hormone receptor signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in azurophil granule membrane	TAS Traceable Author Statement more info
located_in basal part of cell	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	HDA more info	PubMed
located_in ficolin-1-rich granule membrane	TAS Traceable Author Statement more info
located_in intracellular membrane-bounded organelle	IDA Inferred from Direct Assay more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: lysosomal Pro-X carboxypeptidase

Names: angiotensinase C; lysosomal carboxypeptidase C; proline carboxypeptidase

NP_001306143.1

EC 3.4.16.2

NP_005031.1

EC 3.4.16.2

NP_955450.2

EC 3.4.16.2

XP_005274150.1

EC 3.4.16.2

XP_054225298.1

EC 3.4.16.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001319214.2 → NP_001306143.1 lysosomal Pro-X carboxypeptidase isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus and lacks a predicted signal peptide compared to isoform 1.

Source sequence(s)

AA477618, AK302386, AP001646, AW627417, BC001500, BE154586, CB154314

Consensus CDS

CCDS91553.1

UniProtKB/TrEMBL

B7Z7Q6, E9PIG4, E9PNF7, E9PR42

Related

ENSP00000436095.2, ENST00000534264.2

Conserved Domains (1) summary

cl21494
Location:1 → 372

Abhydrolase; alpha/beta hydrolases
NM_005040.4 → NP_005031.1 lysosomal Pro-X carboxypeptidase isoform 1 preproprotein

See identical proteins and their annotated locations for NP_005031.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes isoform 1.

Source sequence(s)

AA477618, AP001646, AW627417, BC001500, BE154586, BI827978, CB154314

Consensus CDS

CCDS8262.1

UniProtKB/Swiss-Prot

A8MU24, B2R7B7, B3KRK5, B5BU34, P42785

UniProtKB/TrEMBL

E9PNF7

Related

ENSP00000317362.3, ENST00000313010.8

Conserved Domains (2) summary

COG0596
Location:104 → 214

MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

cl21494
Location:55 → 477

Abhydrolase; alpha/beta hydrolases
NM_199418.4 → NP_955450.2 lysosomal Pro-X carboxypeptidase isoform 2 precursor

See identical proteins and their annotated locations for NP_955450.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is longer than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1.

Source sequence(s)

AA477618, AK091786, AP001646, AW627417, BC001500, BE154586, CB154314

Consensus CDS

CCDS41695.1

UniProtKB/TrEMBL

E9PNF7

Related

ENSP00000377055.2, ENST00000393399.6

Conserved Domains (2) summary

COG0596
Location:125 → 235

MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

cl21494
Location:78 → 498

Abhydrolase; alpha/beta hydrolases