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Platform GPL10684 Query DataSets for GPL10684
Status Public on Dec 31, 2010
Title NCAUR/USDA-ARS Yeast 70-mer oligo array
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Saccharomyces cerevisiae
Manufacturer NCAUR, USDA-ARS
Manufacture protocol 1. 70-mer universal RNA controls were synthesized with AminoC6 modification at the 5' end
2. Oligos are stored in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
3. Oligos stored at -80℃
4. Allow oligos to thaw at room temperature
5. Shake plate for 1 min
6. Centrifuge plates at 2500 rpm for 1 min
7. Remove seals and cover with plate lids
8. Load the plates in a proper order onto a plate tower
9. Array design with replicated genome in separated blocks
10.Microarray spotting with a humidifier to keep constnet moisture in the chamber
11. Array platform is properly leveled and examined for each direction
12. Printing pins washed with sonication to avoid smaple contamination carry over
 
 
Submission date Jul 14, 2010
Last update date Jan 18, 2013
Contact name Z Lewis Liu
E-mail(s) zlewis.liu@ars.usda.gov
Organization name USDA-ARS
Lab NCAUR
Street address 1815 N University
City Peoria
State/province IL
ZIP/Postal code 61604
Country USA
 
Samples (14) GSM566423, GSM566424, GSM566425, GSM566426, GSM566427, GSM566428 
Series (1)
GSE22939 Comparative transcriptome profiling analyses during the lag phase uncover YAP1, PDR1, PDR3, RPN4 and HSF1 as key regulatory genes in genomic adaptation to the lignocellulose derived inhitibor-stress for saccharomyces cerevisiae

Data table header descriptions
ID
Symbol Gene symbols
Description Gene description
Chromosome Chromosome number
ORF ORF Name
SEQUENCE probe sequence

Data table
ID Symbol Description Chromosome ORF SEQUENCE
Q0010 Q0010 ATATTTATATTATATAATTAATTATATCGTTTATACTCCTTCGGGGTCCCCGCCGGGGCGGGGACTTTAT
Q0017 Q0017 CGTCTCCGAGGTCCCGGTTTCGTAAGAAACCGGGACTTATATATTTATAAATATAAATCTAACTTAATTA
Q0032 Q0032 CTATCCTATATTATCCTATCATATAATATCATATCATATTATATTATATCTTATTATATGATATATAAAGTATTCACTCTATATGAGG
Q0045 COX1 Cox1p MT Q0045 ATGTATTATCAATGGGTGCTATTTTCTCTTTATTTGCAGGATACTATTATTGAAGTCCTCAAATTTTAGG
Q0050 AI1 Ai1p MT Q0050 GTAACTGATCCTTTTGAATATATCGATTCAATTAAATATATATTACCTACAGCTAAAGCTAATTTTAATA
Q0055 AI2 Ai2p MT Q0055 GAAAGTTATTTTAAAATTCGGTAAAGTATTAGTTGATCCTCATTCAAAAGTTAGTTTTAGTATTGATGAT
Q0060 AI3 Ai3p MT Q0060 TAAATGGTAAAAATCGTTCTAGTAGAGCAATGCCTTATTATTGTTTAGAATTAAGACAAAATTATCAAAA
Q0065 AI4 Ai4p MT Q0065 TGTTGGATTTTTTGATGCTGATGGTACAATTAATTATTCATTTAAAAATAATCATCCTCAATTAACAATT
Q0070 AI5_ALPHA Ai5_alphap MT Q0070 ATATGTGATGGTTCATTTGTAAAAGGTGGAGGTTTATATTTAAATTTACAATCTTTTCTAACTAAAGAAT
Q0075 AI5_BETA Ai5_betap MT Q0075 GTATTTTAAAAAGAGATTATAAATCTGGTGCTACAGCTTATATTTATAAAGCTCAATCATCAAAAGCTAT
Q0080 ATP8 Atp8p MT Q0080 GGTTTCTTATTAATGATTCTATTATTAATTTTATTCTCACAATTCTTTTTACCTATGATCTTAAGATTATATGTATCTAG
Q0085 ATP6 Atp6p MT Q0085 AGGTTCTAATATCTTAGCTGGTCATTTATTAATGGTTATTTTAGCTGGTTTACTATTTAATTTTATGTTA
Q0092 Q0092 ATATTCATTATATTTATAATTATATATAATGTAATACGGGTAAACATTACCCGTTGTTCACGGGTAATGT
Q0105 COB Cobp MT Q0105 TCACCTAATACTTTAGGTCATCCTGATAACTATATTCCTGGTAATCCTTTAGTAACACCAGCATCTATTG
Q0110 BI2 Bi2p MT Q0110 TTAGCTTTAGCTATTTGAATTATAGATGATGGATGTAAATTAGGTAAAGGTTTAAAATTCACAACTAATT
Q0115 BI3 Bi3p MT Q0115 GCTGAAAGTTGTTTTAGTATTTATAAACCTATAAATAAAAAAATAAAACTTGCTAGTTTTGAAGTATCTC
Q0120 BI4 Bi4p MT Q0120 AAATTAGACCTCAATTAACTATTAGCGTTACAAATAAATATTTACATGATGTTGAATACTATAGAGAAGT
Q0130 OLI1 F0-ATP synthase subunit c (ATPase-associated proteolipid), encoded on the mitochondrial genome; mutation confers oligomycin resistance; expression is specifically dependent on the nuclear genes AEP1 and AEP2 MT Q0130 TCAAGAAACCCATCAATTAAAGACCTAGTATTCCCTATGGCTATTTTAGGTTTCGCCTTATCAGAAGCTA
Q0140 VAR1 Mitochondrial ribosomal protein of the small subunit, mitochondrially-encoded; polymorphic in different strains due to variation in number of AAT (asparagine) codons; translated near the mitochondrial inner membrane MT Q0140 GTTGGTTGATCTATTAAATTTAAAGGTAGATTAAGTAATAATAATGGTAGAACTAGTACACTTAATTTAT
Q0142 Q0142 GTTCCGGAACTCCTCCTTCTCGCGAGGTTAACACCTATTATATAACTATAACTATAACTATAACTATAAT

Total number of rows: 6398

Table truncated, full table size 1178 Kbytes.




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