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Platform GPL3634 Query DataSets for GPL3634
Status Public on Apr 11, 2006
Title Tor Vergata Mus musculus 200 22q11Ds chip
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer Francesca Amati
Manufacture protocol Each oligonucleotide probe was diluited in a spotting buffer (Corning, Schiphol-rijk, The Netherlands) at a final concentration of 50 pmoli/ul. After this, oliigonucleotide probes were mechanically “spotted” onto UltraGAPS glass slides (Corning, Schiphol-rijk, The Netherlands) using The RoboArrayer™ (Microgrid Compact Plus, BioRobotics ).
Each oligonucleotide probe was represented in triplicate per array. Printing conditions were humidity among 50-55% and temperature 25°C. Printed slides were dried overnight and cross-linked with UV light at 600 mJ using a Strata-linker 2400 (Stratagene, Glenville, VA) and stored in a desiccator at room temperature.
Support glass
 
 
Contributor(s) Biancolella M, Minella D, Novelli G
Submission date Apr 06, 2006
Last update date Apr 11, 2006
Contact name Francesca Amati
E-mail(s) amati@med.uniroma2.it
Phone +39-6-72596080
Organization name University of Rome Tor Vergata
Department Biomedicine and Prevention
Street address Via Montpellier 1
City Roma
ZIP/Postal code 00133
Country Italy
 
Samples (14) GSM111256, GSM111257, GSM111931, GSM111932, GSM111933, GSM111934 
Series (1)
GSE5050 Regulation of 22q11 orthologous genes during mouse development

Data table header descriptions
ID
GB_ACC GenBank accession number
SPOT_ID
Block a collection of circular spot
Row an horizontal sequence of spot
Column a vertical sequence of spot

Data table
ID GB_ACC SPOT_ID Block Row Column
CALIBRATION 1 CALIBRATION 1 1 1 1
CALIBRATION 2 CALIBRATION 2 1 1 2
CALIBRATION 3 CALIBRATION 3 1 1 3
CALIBRATION 4 CALIBRATION 4 1 1 4
CALIBRATION 5 CALIBRATION 5 1 1 5
CALIBRATION 6 CALIBRATION 6 1 1 6
NM_033474 NM_033474 1 2 1
AK004561 AK004561 1 2 2
NM 011239 NM_011239 1 2 3
AF056973 AF056973 1 2 4
EMPTY EMPTY 1 2 5
NM_023348 NM_023348 1 2 6
NM 010048 NM_010048 1 3 1
AF122047 AF122047 1 3 2
NM_010922 NM_010922 1 3 3
AF021031 AF021031 1 3 4
NM_007744 NM_007744 1 3 5
NM016982 NM016982 1 3 6
NM_010104 NM_010104 1 4 1
NM_009170 NM_009170 1 4 2

Total number of rows: 288

Table truncated, full table size 10 Kbytes.






Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided
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