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Platform GPL6796 Query DataSets for GPL6796
Status Public on Jun 20, 2008
Title UT/TH_all-8mer-v1
Technology type in situ oligonucleotide
Distribution custom-commercial
Organism synthetic construct
Manufacturer Agilent Technologies
Manufacture protocol In situ oligosynthesized array with proprietary synthetic sequences that target all possible 8-base sequences. The sequences shown in the below table are not the actual sequences that are printed on the array.
 
Description This protein binding microarray (PBM) involved binding GST-tagged proteins to custom-designed, double-stranded 44K Agilent microarrays in order to determine their sequence preferences. The method is described in Berger et al., Nature Biotechnology 2006. A key feature is that the microarrays are composed of de Bruijn sequences that contain each 10-base sequence once and only once, providing an evenly balanced sequence distribution. Individual de Bruijn sequences have different properties, including representation of gapped patterns. The original Berger et al. array sequences are available via a EULA at http://the_brain.bwh.harvard.edu/UPBMseqn/UPBMseqn_agreement.html.
 
Web link http://the_brain.bwh.harvard.edu/pbms/webworks2/
Citation(s) 16998473
Submission date Apr 29, 2008
Last update date Jan 28, 2009
Contact name Lourdes Pena-Castillo
E-mail(s) lourdes.pena@gmail.com
Phone 416 946-7838
Fax 416 978-8528
Organization name University of Toronto
Department Banting and Best Department of Medical Research
Lab Hughes Lab
Street address 160 College St. Room 1350
City Toronto
State/province Ontario
ZIP/Postal code M5S 3E1
Country Canada
 
Samples (383) GSM285364, GSM285365, GSM285366, GSM285367, GSM285368, GSM285369 
Series (4)
GSE11239 Variation in homeodomain DNA-binding revealed by high-resolution analysis of sequence preferences
GSE12349 A library of yeast transcription factor motifs
GSE34306 Curated collection of yeast transcription factor DNA binding specificity data reveals novel structural and gene regulatory insights

Data table header descriptions
ID 8mer
SEQUENCE 8mer
SEQUENCE 8mer2 (complement strand)

Data table
ID SEQUENCE SEQUENCE
AAAAAAAA AAAAAAAA TTTTTTTT
AAAAAAAC AAAAAAAC GTTTTTTT
AAAAAAAG AAAAAAAG CTTTTTTT
AAAAAAAT AAAAAAAT ATTTTTTT
AAAAAACA AAAAAACA TGTTTTTT
AAAAAACC AAAAAACC GGTTTTTT
AAAAAACG AAAAAACG CGTTTTTT
AAAAAACT AAAAAACT AGTTTTTT
AAAAAAGA AAAAAAGA TCTTTTTT
AAAAAAGC AAAAAAGC GCTTTTTT
AAAAAAGG AAAAAAGG CCTTTTTT
AAAAAAGT AAAAAAGT ACTTTTTT
AAAAAATA AAAAAATA TATTTTTT
AAAAAATC AAAAAATC GATTTTTT
AAAAAATG AAAAAATG CATTTTTT
AAAAAATT AAAAAATT AATTTTTT
AAAAACAA AAAAACAA TTGTTTTT
AAAAACAC AAAAACAC GTGTTTTT
AAAAACAG AAAAACAG CTGTTTTT
AAAAACAT AAAAACAT ATGTTTTT

Total number of rows: 32896

Table truncated, full table size 867 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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