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Status
Public on May 31, 2008
Title
CNIO Mouse chip 27.6K 3.0
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Mus musculus
Manufacturer
Genomic Unit, Biotechnology Program, Spanish National Cancer Research Centre
Manufacture protocol
1.- Individual I.M.A.G.E. Consortium [LLNL] clones were grown in 96-well plates at 37°C for 6 hours. 2.- The bacterial cultures were used for PCR amplification. 3.- PCR products were purified by ultrafiltration, verified by agarose gel electrophoresis and quantified by gel image densitometry. 4.- Probe concentrations were adjusted in 50% DMSO at about 100-150 ng/ul. 5.- Probes were printed onto Gamma Amino Silane Coated glass slides (CMT-GAPS Corning) using the spotter Multigrid II (BioRobotics). 6.- After printing, the slides were baked for 4 hours at 80ºC and stored desiccated in the dark, under vacuum and at room temperature.
Support
glass
Coating
aminosilane
Submission date
May 27, 2008
Last update date
May 31, 2008
Contact name
Rocio G. Urdinguio
E-mail(s)
rgonzalezu@cnio.es
Phone
+34-912246900
Fax
+34-912246923
Organization name
Spanish National Cancer Centre (CNIO)
Department
Molecular Pathology Program
Lab
Cancer Epigenetics
Street address
Melchor Fernandez Almagro 3
City
Madrid
State/province
Madrid
ZIP/Postal code
28029
Country
Spain
Samples (12)
GSM292051 , GSM292052 , GSM292053 , GSM292069 , GSM292070 , GSM292082
Series (1)
GSE11596
Gene expression profiles of wild type and Mecp2-null mice in three different regions of the brain
Data table header descriptions
ID
Block
Each subarray from the main array
Row
Each row from each subarray
Column
Each column from each subarray
CNIO Id
CNIO clone number
Clone ID
Gene Name
Gene name
UG_ID
UniGene cluste clone identifier
GB_ACC
GenBank Accession number
SPOT_ID
CE
Control Elements
Data table
ID
Block
Row
Column
CNIO Id
Clone ID
Gene Name
UG_ID
GB_ACC
SPOT_ID
CE
1
1
1
1
xAA0081
GNOLD43.16
GAPD
Data not found
AJ844644
Positive human
2
1
1
2
mAA0007
H3001A08
Myo1b
Mm.3390
BG062934
3
1
1
3
mAA0205
H3003B02
Myo1b
Mm.3390
BG063103
4
1
1
4
mAA0901
H3010D02
Actb
Mm.297
BG063688
5
1
1
5
xAA0180
GNOLE160
Data not found
Yeast, Spike specific_Cal7.3
Yeast, Spike specific_Cal7.3
6
1
1
6
xAA0182
GNOLE160
Data not found
Yeast, Spike specific_Ratio1.3
Yeast, Spike specific_Ratio1.3
7
1
1
7
xAA0188
GNOLE160
Data not found
Yeast, Spike specific_Ratio3.4
Yeast, Spike specific_Ratio3.4
8
1
1
8
xAA0193
GNOLE160
Data not found
Yeast, Spike specific_Ratio5.5
Yeast, Spike specific_Ratio5.5
9
1
1
9
mAA0101
H3002A06
Lad1
Mm.36726
BG063088
10
1
1
10
mAA0125
H3002C06
Elac2
Mm.157711
BG062943
11
1
1
11
mAA0149
H3002E06
Dbt
Mm.3636
BG062963
12
1
1
12
mAA0173
H3002G06
Tceb2
Mm.153758
BG062985
13
1
1
13
mAA0107
H3002A12
Gpatc2
Mm.138321
BG063094
14
1
1
14
mAA0131
H3002C12
Xrn2
Mm.3065
BG062948
15
1
1
15
mAA0155
H3002E12
Aurkb
Mm.3488
BG062968
16
1
1
16
mAA0179
H3002G12
Data not found
BG062990
17
1
1
17
mAA7781
H3082A06
Mos
Mm.317339
BG070036
18
1
1
18
mAA7805
H3082C06
Sc4mol
Mm.30119
BG070057
19
1
1
19
mAA7829
H3082E06
Pdlim7
Mm.275648
BG070079
20
1
1
20
mAA7853
H3082G06
Arfip1
Mm.316536
BG069934
Total number of rows: 27648 Table truncated, full table size 1594 Kbytes .
Supplementary data files not provided