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Platform GPL8582 Query DataSets for GPL8582
Status Public on Oct 14, 2009
Title MacLeod custom equine cartilage 10K cDNA microarray version 3
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Equus caballus
Manufacturer W.M. Keck Center, University of Illinois Urbana-Champaign
Manufacture protocol The cDNAs were spotted in 3x SSC buffer supplemented with 1.5 M betaine
Support glass
Coating aminosilane
 
Description The 9,322 cDNAs spotted on this microarray were obtained from a normalized and subtracted cDNA library generated from the total RNA of articular cartilage from a 15-month old equine Thoroughbred gelding. In addition, cDNAs from 28 plasmids used within the lab were spotted; 2 plasmids have been submitted to GenBank. Positive control spots include Elongation Factor Tu (EFTu) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Negative control spots are Invitrogen oligos from Arabidopsis thaliana genes CAD1 and GAPC. The original negative control soybean genes chlorophyll ab binding protein (CAB - SPOT_ID: ABbinding) and Rubisco small chain 1 (RBS1 - SPOT_ID: Rubisco) were contaminated prior to spotting with Bos taurus cDNA and thus might show hybridization. Other spots might be identified as empty of buffer. Keywords: cDNA, equine, articular cartilage, microarray
 
Web link http://macleod.uky.edu/equine
Contributor(s) MacLeod JN, Band MR, Sun Q, Mienaltowsk MJ, Coleman SJ
Submission date May 22, 2009
Last update date Oct 14, 2009
Contact name Beatrice Ann McGivney
E-mail(s) beatrice.mcgivney@ucd.ie
Phone 352872852916
Organization name University College Dublin
Department School of Agriculture, Food Science and Veterinary Medicine
Lab Transcriptomics Laboratory
Street address Belfield
City Dublin
State/province Dublin
ZIP/Postal code 4
Country Ireland
 
Samples (32) GSM408853, GSM408854, GSM408855, GSM408856, GSM408857, GSM408858 
Series (1)
GSE16235 Equine skeletal muscle: pre versus post-exercise

Data table header descriptions
ID
Microarray_ID Microarray ID Number
GB_ACC GenBank accession number
SPOT_ID Identifies rows without a GenBank Accession

Data table
ID Microarray_ID GB_ACC SPOT_ID
1 CT010001000A02 CX592099.1
2 CT010001000A03 CX592100.1
3 CT010001000A04 CX592101.1
4 CT010001000A05 CX592102.1
5 CT010001000A06 CX592103.1
6 CT010001000A07 CX592104.1
7 CT010001000A09 CX592106.1
8 CT010001000A10 CX592107.1
9 CT010001000A11 CX592108.1
10 CT010001000A12 CX592109.1
11 CT010001000B01 CX592110.1
12 CT010001000B02 CX592111.1
13 CT010001000B03 CX592112.1
14 CT010001000B05 CX592114.1
15 CT010001000B06 CX592115.1
16 CT010001000B07 CX592116.1
17 CT010001000B08 CX592117.1
18 CT010001000B09 CX592118.1
19 CT010001000B10 CX592119.1
20 CT010001000B12 CX592121.1

Total number of rows: 10070

Table truncated, full table size 296 Kbytes.




Download family Format
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Supplementary data files not provided
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