|
| Status |
Public on Oct 11, 2018 |
| Title |
Centromeres license the mitotic condensation of yeast chromosome arms |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
During mitosis, chromatin condensation shapes chromosomes as separate, rigid and compacted sister-chromatids to facilitate their segregation. Here, we show that unlike wild type yeast chromosomes, non-chromosomal DNA circles and chromosomes lacking a centromere fail to condense during mitosis. Genetics and ChIP-seq experiments establish that the centromere functions in chromosome condensation upstream of the kinases Aurora B and Bub1. Downstream of Aurora B and Bub1, Shugoshin and the deacetylase Hst2 facilitated spreading of the condensation signal from the pericentromeric region to the chromosome arms. Targeting Aurora B to DNA circles or centromere-ablated chromosomes, or releasing Shugoshin from PP2A-dependent inhibition bypassed the centromere requirement for condensation and enhanced the mitotic stability of DNA circles. Our data indicate that yeast cells license in a centromere-dependent manner the chromosome-autonomous condensation of their chromatin, excluding non-centromeric DNA from this process and thereby inhibiting their propagation.
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| |
|
| Overall design |
Analysis of histone modification in different experimental conditions
|
| |
|
| Contributor(s) |
Chymkowitch P, Kruitwagen T, Enserink J, Barral Y |
| Citation(s) |
30318142 |
| |
| Submission date |
Jun 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Pierre Chymkowitch |
| E-mail(s) |
pierrech@ibv.uio.no
|
| Organization name |
University of Oslo
|
| Department |
Biosciences
|
| Lab |
Chymkowitch
|
| Street address |
Blindernveien 31
|
| City |
Oslo |
| ZIP/Postal code |
0371 |
| Country |
Norway |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (51)
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|
| Relations |
| BioProject |
PRJNA392397 |
| SRA |
SRP110747 |
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