|
| Status |
Public on Jan 20, 2018 |
| Title |
RNA seq of pancreatic cancer-associated fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptomic profiling of pancreatic cancer-associated fibroblasts (CAFs) was done to evaluate the expression of GPCRs. The same was also done for normal pancreatic stellate cells, so as to evaluate whether a population of GPCRs shows increased expression in CAFs vs their normal precursors. We report the discovery of a novel GPCR in CAFs; we demonstrate this GPCR helps to drive the cross-talk between CAFs and cancer cells, enhancing the growth of pancreatic cancer cells.
|
| |
|
| Overall design |
Stranded mRNA sequencing of 3 lines of de-identified patient-derived, low-passage pancreatic CAFs and 1 line of normal pancreatic stellate cells (PSCs)
|
| |
|
| Contributor(s) |
Wiley SZ, Sriram K, Insel PA |
| Citation(s) |
29092903, 31646252 |
| |
| Submission date |
Jul 20, 2017 |
| Last update date |
Oct 28, 2019 |
| Contact name |
Paul A Insel |
| Phone |
858 534 2298
|
| Organization name |
UC San Diego
|
| Department |
Pharmacology
|
| Lab |
Insel
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (4)
|
| GSM2711739 |
Pancreatic cancer-associated fibroblasts, 2 |
| GSM2711740 |
Pancreatic cancer-associated fibroblasts, 3 |
| GSM2711741 |
Pancreatic cancer-associated fibroblasts, 5 |
|
| Relations |
| BioProject |
PRJNA395149 |
| SRA |
SRP113156 |
Less...
More...