|
| Status |
Public on Oct 14, 2019 |
| Title |
Heterozygous SNORA31 mutations in patients with herpes simplex encephalitis |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Pseudo-seq was used to measure differences in the extent of pseudouridine (Ψ) modification in rRNA from human cell lines with heterozygous, and homozygous disruptions of SNORA31.
|
| |
|
| Overall design |
CMC treatment of RNA followed by next generation sequencing was used to measure the extent of pseudouridylation at mapped human rRNA Ψ sites.
CMC: carbodiimide N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate
|
| Web link |
https://www.nature.com/articles/s41591-019-0672-3
|
| |
|
| Contributor(s) |
Lafaille FG, Casanova J, Carlile TM, Gilbert WV, Zhang S |
| Citation(s) |
31806906 |
| |
| Submission date |
Jul 31, 2017 |
| Last update date |
Jan 13, 2020 |
| Contact name |
Thomas M Carlile |
| E-mail(s) |
tcarlile@gmail.com
|
| Phone |
6172533707
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Gilbert
|
| Street address |
77 Massachusetts Ave
|
| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA396550 |
| SRA |
SRP114432 |
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