|
| Status |
Public on Sep 15, 2017 |
| Title |
Single-cell RNA-seq analysis of human CD14+ monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed single-cell RNA-seq on CD14+ monocytes isolated from the blood of healthy donors. Using the 10x chromium technology, we analyzed 425 and 431 cells from 2 individual donors.
|
| |
|
| Overall design |
Peripheral Blood Mononuclear Cells (PBMC) were prepared by centrifugation on a Ficoll gradient. Blood CD14+ monocytes were isolated from healthy donors’ PBMC by positive selection using magnetic beads. Monocytes were 93-95% CD14+CD16- as assessed by flow cytometry. Cellular suspensions (1700 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer’s protocol.
|
| |
|
| Contributor(s) |
Segura E, Tang-Huau T, Bohec M, Gueguen P, Goudot C |
| Citation(s) |
28930664 |
| |
| Submission date |
Sep 06, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Elodie Segura |
| E-mail(s) |
elodie.segura@curie.fr
|
| Phone |
+336156246720
|
| Organization name |
Institut Curie
|
| Department |
INSERM U932
|
| Street address |
26 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA401805 |
| SRA |
SRP117082 |
