|
| Status |
Public on May 31, 2018 |
| Title |
RNA-Seq analysis of localization along animal-vegetal axis of Xenopus laevis |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We combined cryosectining of oocytes along the animal-vegetal axis (first developmental axis) and RNA-Seq to determine localization profiles of coding and noncoding RNAs. It provides complete view on RNA localization. We found that nearly all RNAs are localized, but only small percentage is actively transported during oogenesis.
|
| |
|
| Overall design |
RNA profiles (mRNA, lncRNA, other RNA) were generated by deep sequencing, in biological triplicates (each oocyte divided into five segments), using Illumina HiSeq.
|
| |
|
| Contributor(s) |
Sindelka R, Abaffy P |
| Citation(s) |
29844480, 32514328 |
| |
| Submission date |
Oct 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Radek Šindelka |
| Organization name |
Institute of Biotechnology, Czech Academy of Science, v.v.i.
|
| Lab |
Laboratory of Gene Expression
|
| Street address |
Průmyslová 595
|
| City |
Vestec by Prague |
| ZIP/Postal code |
25250 |
| Country |
Czech Republic |
| |
|
| Platforms (1) |
| GPL17682 |
Illumina HiSeq 2000 (Xenopus laevis) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA413987 |
| SRA |
SRP119791 |
Less...
More...