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| Status |
Public on Dec 20, 2017 |
| Title |
MicroRNA differencial expression data between scrambled siRNA-treated mice and HN1L siRNA-treated mice tumor samples |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
(HN1L) is a targetable breast cancer stem cell (BCSC) gene that is altered in 25% of whole breast cancer and significantly correlated with shorter overall or relapse-free survival in triple negative breast cancer (TNBC) patients. HN1L silencing reduced the population of BCSCs, inhibited tumor initiation, re-sensitized chemo-resistant tumors to docetaxel, and hindered cancer progression in multiple TNBC cell line derived xenografts.
We used miRNA array to compare miRNA expression profiles from PDX BCM2665 xenograft tumors with or without HN1L knockdown, to identify role of HN1L in regulating miRNA.
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| Overall design |
miRNA arrays were performed on 10 snap-frozen tumor samples from scrambled siRNA-treated and HN1L siRNA-treated BCM2665 xenografts (5 samples from each treatment group). Differencial expression was performed to identifiy significantly differencially expressed miRNA and corresponding pathways.
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| |
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| Contributor(s) |
Chang JC, Elemento O, Verma A, Liu Y, Choi DS, Wong H |
| Citation(s) |
29249663 |
| |
| Submission date |
Oct 26, 2017 |
| Last update date |
Mar 21, 2018 |
| Contact name |
Jenny Chang |
| Organization name |
Houston Methodist Hospital
|
| Department |
Houston Methodist Cancer Center
|
| Street address |
6670 Bertner Ave.
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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| Samples (10)
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| GSM2831501 |
tumor from scrambled siRNA-treated mouse #4817 [miRNA] |
| GSM2831502 |
tumor from scrambled siRNA-treated mouse #4819 [miRNA] |
| GSM2831503 |
tumor from scrambled siRNA-treated mouse #4959 [miRNA] |
| GSM2831504 |
tumor from scrambled siRNA-treated mouse #4967 [miRNA] |
| GSM2831505 |
tumor from scrambled siRNA-treated mouse #4989 [miRNA] |
| GSM2831506 |
tumor from HN1L siRNA-treated mouse #4809 [miRNA] |
| GSM2831507 |
tumor from HN1L siRNA-treated mouse #4810 [miRNA] |
| GSM2831508 |
tumor from HN1L siRNA-treated mouse #4814 [miRNA] |
| GSM2831509 |
tumor from HN1L siRNA-treated mouse #4950 [miRNA] |
| GSM2831510 |
tumor from HN1L siRNA-treated mouse #4984 [miRNA] |
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| This SubSeries is part of SuperSeries: |
| GSE106200 |
RNA differencial expression data between scrambled siRNA-treated mice and HN1L siRNA-treated mice tumor samples |
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| Relations |
| BioProject |
PRJNA415910 |
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