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Status |
Public on Nov 09, 2017 |
Title |
Transcriptome change caused by lowered intracellular S-adenosylmethionine level in the X63/0 mouse plasma cell line. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We aimed to examine the gene expression changes responding to a depletion of intracellular S-adenosylmethionine (SAM). The mouse plasma cell line X63/0 was treated with the SAM-synthetase inhibitor cycloleucine (cLEU), and the total RNA was isolated and analyzed by RNA-sequencing. As a result, we idntified 27 genes, including the ubiquitous SAM-synthetase MAT2A, whose expssions were up-regulated by two-fold or more.
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Overall design |
Total RNA was extracted from the mouse plasma cell line X63/0 before/after a three-hour treatment with 30 mM cycloleucine. This experiment was triplicated, and the resulting six samples were applied to RNA-sequencing.
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Contributor(s) |
Matsumoto M, Shima H, Igarashi K |
Citation(s) |
29262316 |
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Submission date |
Nov 08, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Mitsuyo Matsumoto |
E-mail(s) |
mitsuyo.matsumoto.d5@tohoku.ac.jp
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Organization name |
Tohoku university school of medicine
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Department |
Biochemistry
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Street address |
2-1 Seiryo-machi Aoba-ku
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City |
Sendai |
ZIP/Postal code |
980-8575 |
Country |
Japan |
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Platforms (1) |
GPL18635 |
Ion Torrent Proton (Mus musculus) |
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Samples (2) |
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Relations |
BioProject |
PRJNA417590 |
SRA |
SRP124606 |