|
| Status |
Public on Dec 05, 2017 |
| Title |
E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [RNA-seq] |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The purpose of this experiment was to characterize MazF cleavage specificity and locations of cleavage genome-wide in E. coli and also identify any leaderless transcripts .To do this, MazF was expressed in a ΔmazF background and compared to an empty vector. rRNA-subtracted samples were used to assess cleavage in the mRNA, and total RNA samples were used to assess changes in the rRNA.
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| |
|
| Overall design |
Two replicates of + MazF samples and empty vector samples after 5 minutes of induction (rRNA subtracted), two replicates of + MazF and empty vector samples after 5, 30 and 60 minutes (total RNA).
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| |
|
| Contributor(s) |
Culviner PH, Laub MT |
| Citation(s) |
29861158 |
| |
| Submission date |
Nov 24, 2017 |
| Last update date |
Sep 05, 2021 |
| Contact name |
Peter Culviner |
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Laub
|
| Street address |
31 Ames St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
|
| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE107330 |
E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis |
|
| Relations |
| BioProject |
PRJNA419767 |
| SRA |
SRP125610 |
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