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Status |
Public on May 08, 2008 |
Title |
Liver Genomic Responses to Ciguatoxin (P-CTX-1) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Ciguatoxins (CTX) are polyether neurotoxins responsible for ciguatera, the most common fish-borne food poisoning in humans. This study characterizes the global transcriptional response of mouse liver to a symptomatic dose (0.26 ng/g) of the highly potent Pacific ciguatoxin-1 (P-CTX-1). At 1 h post exposure 2.4% of features on a 44K whole genome array were differentially expressed (p ≤ 0.0001), increasing to 5.2% at 4 h and decreasing to 1.4% by 24 h post-CTX exposure. Early gene expression was likely influenced prominently by an acute 4 °C decline in core body temperature by 1 h, which resolved by 8 h following exposure. Cytochrome P450s were of particular interest due to their role in xenobiotic metabolism. Twenty-seven genes, mostly members of Cyp2 and Cyp4 families, showed significant changes in expression. Many Cyps underwent an initial down-regulation at 1 h but were quickly and strongly up-regulated at 4 and 24 h post exposure. In addition to Cyps, increases in several glutathione S-transferases were observed, an indication that both phase I and phase II metabolic reactions are involved in the hepatic response to CTX in mice. Keywords: biotoxin, time course
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Overall design |
Individual C57/BL6 mice (n=3) exposed to 0.26 ng/g P-CTX-1 ip for 1, 4, or 24 h were compared to pooled time-matched controls (n=3). The triplicate arrays at each time point included a dye swap.
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Contributor(s) |
Morey JS, Ryan JC, Van Dolah FM |
Citation(s) |
18353800 |
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Submission date |
Mar 09, 2008 |
Last update date |
Feb 11, 2020 |
Contact name |
Frances M Van Dolah |
E-mail(s) |
fran.vandolah@noaa.gov
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Organization name |
Marine Biotoxins Program, NOAA Center for Coastal Environmental Health and Biomolecular Research
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Street address |
219 Fort Johnson Rd
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City |
Charleston |
State/province |
SC |
ZIP/Postal code |
29412 |
Country |
USA |
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Platforms (1) |
GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
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Samples (9)
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GSM271872 |
Liver_Ciguatoxin (P-CTX-1)_4h_513- |
GSM271873 |
Liver_Ciguatoxin (P-CTX-1)_4h_512+ |
GSM271874 |
Liver_Ciguatoxin (P-CTX-1)_4h_715- |
GSM271875 |
Liver_Ciguatoxin (P-CTX-1)_24h_051- |
GSM271876 |
Liver_Ciguatoxin (P-CTX-1)_24h_052+ |
GSM271877 |
Liver_Ciguatoxin (P-CTX-1)_24h_050+ |
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Relations |
BioProject |
PRJNA107559 |