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| Status |
Public on Feb 19, 2020 |
| Title |
Increased intron retention is a post-transcriptional signature associated with progressive ageing and Alzheimer’s disease |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Intron retention (IR) may affect gene expression and protein functions during development and age-onset diseases. However, it remains unclear if IR undergoes spatial or temporal changes during different stages of ageing or neurodegenerative disorders like Alzheimer’s disease (AD). By profiling mRNA species across different ages of Drosophila heads, we observed significant increase in the level of IR of many conserved genes as animals aged. Interestingly, distinct sets of genes are affected at different stages of adult fly life with IR occurring at several AD-associated genes in old adult. This suggests that alteration of proper protein functions by IR during ageing may lead to AD pathogenesis. Consistent with this notion, analyses of healthy human ageing brains and different AD datasets revealed similar increased aberrant IR activities in many AD-curated genes. Taken together, our data suggest that increase IR during ageing may be the driver for late-onset sporadic AD.
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| Overall design |
Examination the transcriptome (by RNA-sequencing) of Drosophila heads isolated at day 10, 20, 30 and 50 of adult life.
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| Contributor(s) |
Ong C, Adusumalli S |
| Citation(s) |
30868713 |
| |
| Submission date |
Feb 08, 2018 |
| Last update date |
Feb 19, 2020 |
| Contact name |
Swarnaseetha Adusumalli |
| E-mail(s) |
swarna@tll.org.sg
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| Organization name |
Temasek Life Sciences Laboratory
|
| Street address |
1 Research Link, National University of Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
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| Platforms (1) |
| GPL21306 |
Illumina HiSeq 4000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA433513 |
| SRA |
SRP132479 |
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