 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 14, 2008 |
| Title |
Colony Stimulating Factor 1 (CSF-1) responses in human monocyte derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Although the role of macrophage colony stimulating factor (M-CSF/CSF-1) in homeostasis and disease processes has been studied extensively in mice, little is known of the impact of this cytokine on differentiated human macrophages. Here we show that, in contrast to its effects on mouse bone marrow-derived macrophages (BMM), CSF-1 did not induce expression of urokinase plasminogen activator mRNA, repress expression of apolipoprotein E mRNA, or prime LPS-induced TNF secretion in human monocyte-derived macrophages (HMDM) from several independent donors. Using expression profiling, we show that CSF-1 dynamically regulated the expression of several genes that encode chemokines and chemokine receptors (e.g. CXCL10/IP-10, CXCL2, CCL7, SDF2L1, CXCR4) in HMDM. CSF-1 also upregulated the expression of several genes encoding enzymes of the cholesterol biosynthetic pathway (HMGCR, MVD, IDI1, FDPS, SQLE, CYP51A1, EBP, NSDHL, DHCR7 and DHCR24), while expression of ABCG1, encoding a cholesterol efflux transporter, was repressed. Although the CSF-1/CSF-1R system has been proposed as a target for the treatment of inflammatory and metastatic disease based on studies in rodents, this is the first systematic analysis of the effects of CSF-1 on mature human macrophages. Our data demonstrates that CSF-1 represents a further link between inflammation and cardiovascular disease, inflammtion and immunity.
Keywords: Stimulus response
|
| |
|
| Overall design |
In vitro differentiated (HMDM) from 3 individuals were stimulated with CSF-1 for 6h and compared to unstimulated controls on a single-channel microarray.
|
| |
|
| Contributor(s) |
Irvine KM, Sweet MJ, Hume DA |
| Citation(s) |
19004987 |
| |
| Submission date |
Apr 04, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Katharine Irvine |
| E-mail(s) |
k.irvine@imb.uq.edu.au, m.sweet@imb.uq.edu.au
|
| Organization name |
Institute for Molecular Bioscience
|
| Street address |
306 Carmody Rd
|
| City |
Brisbane |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL2507 |
Sentrix Human-6 Expression BeadChip |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA107049 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11061_RAW.tar |
7.7 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...