|
| Status |
Public on Mar 01, 2019 |
| Title |
Gene expression changes in A375 melanoma cells in response to ERK3 knockdown. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression microarrays were used to compare A375 cells treated with a control siRNA or an siRNA targeting ERK3 (MAPK6).
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| |
|
| Overall design |
Three replicates are included for each condition (siCon and siERK3). Total RNA was extracted using Trizol® reagent (Ambion) three days post-siRNA-transfection. RNA quality was assessed on an Agilent 2100 Bioanalyzer following the manufacturer’s protocol. Samples with RNA integrity numbers (RIN) >6 were used for further analysis. RNA concentration was measured by NanoDrop™ One Spectrophotometer (Thermo Fisher Scientific). Affymetrix microarray system was used for gene expression analysis using Clariom™ S Pico Assay (human) platform with WT PLUS Kit and GeneChip® Hybridization, Wash, and Stain Kit following the manufacturer’s protocols.
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| |
|
| Contributor(s) |
Markey MP |
| Citation(s) |
30569573 |
| |
| Submission date |
Mar 19, 2018 |
| Last update date |
Jan 06, 2020 |
| Contact name |
Michael Markey |
| E-mail(s) |
michael.markey@wright.edu
|
| Phone |
937-775-4536
|
| Fax |
937-775-3031
|
| URL |
http://www.med.wright.edu/cgr/
|
| Organization name |
Wright State Unviersity
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Center for Genomics Research
|
| Street address |
3640 Colonel Glenn Hwy.
|
| City |
Dayton |
| State/province |
OH |
| ZIP/Postal code |
45435 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
|
| Samples (6)
|
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| Relations |
| BioProject |
PRJNA438995 |
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