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Status |
Public on Apr 10, 2020 |
Title |
Translin KO mice display elevated adiposity |
Platform organism |
synthetic construct |
Sample organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
The high prevalence of obesity has focused attention on defining the pathophysiological processes that underlie susceptibility or resistance to its deleterious metabolic consequences. Mice lacking translin (Tsn), a gene implicated in a variety of biological functions from transcription to microRNA degradation, display extremely high levels of adiposity, comparable to those found in well-known genetic models of obesity, such as melanocortin 4 receptor or leptin knockout (KO) mice. Although translin KO mice display increased adiposity they retain normal glucose tolerance. In contrast, wild-type (WT) mice placed on a high-fat diet until they match translin KO adiposity levels are glucose intolerant, as expected. Conversely, translin KO mice display prominent hepatic steatosis that is more severe than that of adiposity-matched WT mice. The ability of translin KO mice to retain normal glucose tolerance in the face of massive tissue expansion may be due to three factors: preferential accumulation of subcutaneous fat, reduced levels of TNF mRNA in both adipose and hepatic tissue, and elevated levels of plasma adiponectin. Further studies aimed at defining the molecular bases for these phenotypes may yield new approaches to limit the adverse metabolic consequences of obesity.
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Overall design |
Comparison between adipose tissue microRNA expression levels from adult, male translin KO mice and wildtype (WT) mice with six biological replicates per group.
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Contributor(s) |
Baraban JM, Shah A, Fu X, Talbot CC Jr |
Citation(s) |
30647452 |
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Submission date |
May 25, 2018 |
Last update date |
Apr 10, 2020 |
Contact name |
Jay M Baraban |
Organization name |
The Johns Hopkins University School of Medicine
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Department |
Neuroscience
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Lab |
WBSB 908
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Street address |
725 North Wolfe Street
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City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21205 |
Country |
USA |
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Platforms (1) |
GPL19117 |
[miRNA-4] Affymetrix Multispecies miRNA-4 Array |
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Samples (12)
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Relations |
BioProject |
PRJNA473148 |