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Status |
Public on Oct 30, 2018 |
Title |
Genome-wide maps of ParBF (SopB) binding on plasmid F or E.coli chromosome carrying parSF |
Organism |
Escherichia coli |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We report the genome-wide analysis from chromatin immunoprecipitated DNA (ChIP-sequencing) at very high resolution of the DNA binding pattern of ParBF (SopB) either on the full length plasmid F or on E. coli chromosome carrying the parSF centromere sequence. We also varied the intracellular ParBF concentration to discriminate between the several proposed mechanism of partition complexes assembly.
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Overall design |
Differencial examination of ParBF binding by comparing input and IP (affinity-purified anti-ParBF antibody) samples
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Contributor(s) |
Diaz RE, Bouet J, Labourdette D |
Citation(s) |
30446599 |
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Submission date |
Jun 04, 2018 |
Last update date |
Jan 29, 2019 |
Contact name |
Delphine Labourdette |
E-mail(s) |
labourd@insa-toulouse.fr
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Organization name |
INSA
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Department |
LISBP
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Lab |
Plateforme Biopuces
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Street address |
Hall Gilbert Durand - Bio5 - 135 Avenue de Rangueil
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City |
Toulouse |
ZIP/Postal code |
31140 |
Country |
France |
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Platforms (2) |
GPL21726 |
Ion Torrent Proton (Escherichia coli) |
GPL25140 |
Ion Torrent S5 (Escherichia coli) |
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Samples (29)
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Relations |
BioProject |
PRJNA474445 |
SRA |
SRP149674 |