|
| Status |
Public on Oct 30, 2018 |
| Title |
Genome-wide maps of ParBF (SopB) binding on plasmid F or E.coli chromosome carrying parSF |
| Organism |
Escherichia coli |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the genome-wide analysis from chromatin immunoprecipitated DNA (ChIP-sequencing) at very high resolution of the DNA binding pattern of ParBF (SopB) either on the full length plasmid F or on E. coli chromosome carrying the parSF centromere sequence. We also varied the intracellular ParBF concentration to discriminate between the several proposed mechanism of partition complexes assembly.
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| |
|
| Overall design |
Differencial examination of ParBF binding by comparing input and IP (affinity-purified anti-ParBF antibody) samples
|
| |
|
| Contributor(s) |
Diaz RE, Bouet J, Labourdette D |
| Citation(s) |
30446599 |
| |
| Submission date |
Jun 04, 2018 |
| Last update date |
Jan 29, 2019 |
| Contact name |
Delphine Labourdette |
| E-mail(s) |
labourd@insa-toulouse.fr
|
| Organization name |
INSA
|
| Department |
LISBP
|
| Lab |
Plateforme Biopuces
|
| Street address |
Hall Gilbert Durand - Bio5 - 135 Avenue de Rangueil
|
| City |
Toulouse |
| ZIP/Postal code |
31140 |
| Country |
France |
| |
|
| Platforms (2) |
| GPL21726 |
Ion Torrent Proton (Escherichia coli) |
| GPL25140 |
Ion Torrent S5 (Escherichia coli) |
|
| Samples (29)
|
|
| Relations |
| BioProject |
PRJNA474445 |
| SRA |
SRP149674 |
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