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| Status |
Public on Nov 27, 2018 |
| Title |
Similarities and differences in the regulation of HoxD genes during chick and mouse limb development [RNA-Seq] |
| Organisms |
Gallus gallus; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Hoxd genes are important for limb development in all tetrapods examined thus far. In mouse, they are controlled by a complex bimodal regulation, which regulates first the proximal patterning, then the distal structure, allowing at the same time the formation of the wrist and ankle articulations. We analyzed the existence of this regulatory mechanism in chicken, i.e. in an animal where large morphological differences exist between fore- and hindlimbs. We report that while this bimodal regulation is globally conserved between mammals and avian, some important modifications evolved at least between these two model systems, in particular regarding the activity of specific enhancer and the position of the TAD boundary and the forelimb versus hindlimb comparative regulations. Some aspects of these regulations seem to be more conserved between chick and bats than with the mouse situation, which may relate to the extent to which forelimbs and hindlimbs of these various animals differ in their functions.
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| |
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| Overall design |
RNA-seq analysis of limb tissues from mouse (E12.5) or chick (HH20 and HH30)
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| Contributor(s) |
Yakushiji-Kaminatsui N, Beccari L, Necsulea A, Lopez-Delisle L, Duboule D |
| Citation(s) |
30475793 |
| |
| Submission date |
Jun 11, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Nayuta Yakushiji-Kaminatsui |
| E-mail(s) |
nayuta.yakushiji@riken.jp
|
| Phone |
+81 45 503 9690
|
| Organization name |
RIKEN Center for Integrative Medical Sciences
|
| Lab |
Laboratory for Developmental Genetics
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platforms (3) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE115563 |
Similarities and differences in the regulation of HoxD genes during chick and mouse limb development. |
|
| Relations |
| BioProject |
PRJNA475487 |
| SRA |
SRP150197 |
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