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| Status |
Public on Aug 21, 2018 |
| Title |
Characterizing the gene expression profile of Prox1+ intestinal adenoma organoid cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We isolated and selected intestinal adenoma organoids from Apcmin/+; Rosa26LSL-TdTomato; Prox1-CreERT2 mice. After the selection procedure without growth factors, we induced CreERT2 activity and the transcription of tdTomato to label Prox1+ cells by 300 nM 4-hydroxytamoxifen for 16h. tdTomato+ (Prox1+) and tdTomato- cells (enriched for Prox1- cells) were FACS sorted and total RNA was isolated.
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| Overall design |
Total RNA obtained from tdTomato+ (Prox1+) cells was compared to the tdTomato- cell population (enriched for Prox1- cells) after FACS sorting.
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| Contributor(s) |
Högström J, Wiener Z, Alitalo K |
| Citation(s) |
30154153 |
| |
| Submission date |
Aug 01, 2018 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Zoltan Wiener |
| E-mail(s) |
zoltan.wiener@gmail.com
|
| Organization name |
Semmelweis University
|
| Street address |
Ulloi ut 26
|
| City |
Budapest |
| ZIP/Postal code |
H-1085 |
| Country |
Hungary |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA483896 |
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