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| Status |
Public on May 17, 2019 |
| Title |
Guanidineacetic acid regulate myogenic differentiation through miR-133a-5 and miR-1a-3p co-mediated PI3K-Akt-mTOR signaling pathway |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Through small RNA sequencing, we finded that a total of 8 miRNAs, including miR-133a-3p and miR-1a-3p cluster, showed differential expression after guanidineacetic acid supplement. To further study the function of miR-133a-3p and miR-1a-3p in guanidineacetic acid induce myotube hypertrophy, we transfected miR-133a-3p and miR-1a-3p mimics, that also induce myotube hypertrophy. Through bioinformatics and dual-luciferase reporter system, the target gene of miR-133a-3p and miR-1a-3p were respectively determined. Meanwhile, miR-133a-3p and miR-1a-3p modulate PI3K-Akt-mTOR signaling pathway by restraining target gene expression.
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| Overall design |
We used small RNA sequencing to detect miRNA transcriptomes of myotubes after 20 mM guanidineacetic acid treatment (GAA) or nagtive control(NC) (2 vs. 2).
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| Contributor(s) |
Wang Y, Ma J |
| Citation(s) |
30235878 |
| |
| Submission date |
Aug 14, 2018 |
| Last update date |
May 17, 2019 |
| Contact name |
wang yu jie |
| E-mail(s) |
wangyujie715@163.com
|
| Phone |
+8218227586326
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| Organization name |
Sichuan Agricultural University
|
| Street address |
211 Huimin Road, Wenjiang District, Chengdu
|
| City |
chengdu |
| ZIP/Postal code |
611130 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA485944 |
| SRA |
SRP157904 |
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