|
| Status |
Public on Mar 26, 2019 |
| Title |
Single cell RNA-seq of Epidermal cells from newborn (P1) wildtype and BAP1 knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
BAP1 deletion in primary mouse epidermal cell culture leads to increased melanocytes population. However, it is unclear whether BAP1 exhibits its function in melanocytes or in the progenitor cells for melanocytes, since the epidermal culture contains a mixed cell types. We aim to carry out a single cell RNAseq profiling with this system, and dissect BAP1's roles in different cell types.
|
| |
|
| Overall design |
Epidermal cells from newborn (P1) mice after culture for 7 days ex vivo - one wildtype (BAP1 WT) and one BAP1 knockout (BAP1 KO) sample were dissociated into single cells and sequenced.
|
| |
|
| Contributor(s) |
He M, Reja R |
| Citation(s) |
31000662 |
| |
| Submission date |
Sep 25, 2018 |
| Last update date |
Apr 22, 2019 |
| Contact name |
Rohit Reja |
| E-mail(s) |
rejar@gene.com
|
| Phone |
+1-650-467-7615
|
| Organization name |
Genentech
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE120447 |
Intrinsic Apoptosis Shapes the Tumor Spectrum Linked to Inactivation of the Deubiquitinase BAP1 |
|
| Relations |
| BioProject |
PRJNA493026 |
| SRA |
SRP162525 |
