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| Status |
Public on Nov 01, 2018 |
| Title |
Translatome and transcriptome analysis of TMA20 (MCT-1) and TMA64 (eIF2D) knockout yeast strains |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We report ribosome profiling and RNA-Seq data of S.cerevisiae strains with TMA20, TMA64 single- and TMA20/TMA64 double-gene knockouts, grown in the rich YPD or minimal SD medium.
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| Overall design |
Yeast cells of 4 different S. cerevisiae strains, namely: Δtma20, Δtma64, ΔΔtma20tma64 knockout strains and the wild-type BY4741, were maintained in YPD or SD media. Two biological replicates were performed for wild-type strain, maintained in YPD media. Ribosomal profiling experiments of the wild-type and knockout strains grown in different media were performed. The сDNA libraries were obtained from total 9 RNA samples and 9 ribosome-bound RNA samples.
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| Contributor(s) |
Makeeva DS, Logacheva MD, Dmitriev SE, Kulakovskiy IV |
| Citation(s) |
30815525 |
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| Submission date |
Oct 31, 2018 |
| Last update date |
Mar 13, 2019 |
| Contact name |
Ivan V Kulakovskiy |
| Organization name |
Institute of Protein Research
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| Street address |
Institutskaya 4
|
| City |
Pushchino |
| ZIP/Postal code |
142290 |
| Country |
Russia |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA503203 |
| SRA |
SRP167363 |
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