|
| Status |
Public on Mar 01, 2019 |
| Title |
Expansion of bacteriophages during disease worsens intestinal inflammation and colitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purified phage was used to prevent tumor growth in a mouse model of bacteria aggravated-colorectal cancer. Composite E. coli phage or vehicle control was added to the drinking water of specific pathogen free (SPF) APCmin mice and animals were colonized with E.coli NC101. APCmin mice displayed no overall difference in the number of tumors that formed within the small intestine, however colonization with E. coli NC101 accelerated the growth of tumors resulting in a significant increase in large tumor formation. Importantly, bacteriophage treatment of AIEC colonized APCmin animals significantly reduced E. coli colonization.
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| Overall design |
4 week old APCmin heterozygous mice were given a cocktail of 3 E. coli phage in drinking water for 4 weeks, then challenged with E. coli NC101 via oral gavage biweekly for 2 weeks. Mice were then maintained on drinking water containing phage mixture for 2 months.
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| |
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| Contributor(s) |
Gogokhia L, Stephens Z |
| Citation(s) |
30763538 |
| |
| Submission date |
Dec 19, 2018 |
| Last update date |
May 31, 2019 |
| Contact name |
Chris Stubben |
| Organization name |
Huntsman Cancer Institute
|
| Department |
University of Utah
|
| Street address |
2000 Cir of Hope Dr
|
| City |
Salt Lake City |
| State/province |
Utah |
| ZIP/Postal code |
84103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA510809 |
| SRA |
SRP173935 |
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