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| Status |
Public on Oct 01, 2008 |
| Title |
Origin and pathogenesis of lymphocyte-predominant Hodgkin lymphoma as revealed by global gene expression analysis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The pathogenesis of nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) and its relationship to other lymphomas are largely unknown. This is partly due to the technical challenge of analyzing its rare neoplastic L&H cells, which are dispersed in an abundant non-neoplastic cellular microenvironment. We performed a genome-wide expression study of microdissected lymphocytic and histiocytic (L&H) lymphoma cells in comparison to normal and other malignant B cells, which indicates a relationship of L&H cells to and/or origin from germinal center B cells at transition to memory B cells. L&H cells show a surprisingly high similarity to the tumor cells of T cell-rich B cell lymphoma and classical Hodgkin lymphoma, a partial loss of their B cell phenotype and deregulation of many apoptosis-regulators and putative oncogenes. Importantly, L&H cells are characterized by constitutive NF-κB activity and aberrant ERK signaling. Thus, these findings shed new light on the nature of L&H cells, revealed several novel pathogenetic mechanisms in NLPHL, and may help in differential diagnosis and lead to novel therapeutic strategies.
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| Overall design |
Analysis of differential gene expression in primary human lymphoma cells of nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) in comparison with primary lymphoma cells of classical Hodgkin lymphoma cells and other B-non-Hodgkin lymphoma (B-NHL) samples and subsets of non-neoplastic B lymphocytes isolated from blood or tonsils. 67 gene expression profiles were analysed.
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| Contributor(s) |
Brune V, Tiacci E, Pfeil I, Döring C, Eckerle S, van Noesel C, Klapper W, Falini B, von Heydebreck A, Metzler D, Bräuninger A, Hansmann M, Küppers R |
| Citation(s) |
18794340, 24376854, 29889102 |
| |
| Submission date |
Aug 14, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Ralf Küppers |
| E-mail(s) |
ralf.kueppers@uk-essen.de
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| Phone |
0049 201 723 3384
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| Fax |
0049 201 723 3386
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| Organization name |
University of Duisburg-Essen, Medical School
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| Department |
Institute for Cell Biology (Tumor Research)
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| Street address |
Virchowstr. 173
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| City |
Essen |
| ZIP/Postal code |
45122 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (67)
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| Relations |
| BioProject |
PRJNA113011 |
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