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| Status |
Public on Dec 31, 2020 |
| Title |
Expression data from transdetermined Drosophila eye antennal imaginal disc by vestigial and simjang overexpression. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Drosophila imaginal disc cells exhibit a remarkable ability to switch cell fates under various perturbations, a phenomenon known as transdetermination (TD). The process of cell reprogramming seems to be regulated in stochastic manners. Ectopic expression of the master transcription factor of wing identity, vestigial, in eye progenitor cells induces eye-to-wing TD, but the frequency of the TD is low. We found that the overexpression of simjang gene dramatically enhanced eye-to-wing TD frequency. Gene network controlling this process, however, is largely unclear.
We used microarray to detail the global gene network underlying simj-mediated facilitation of eye-to-wing TD.
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| Overall design |
Third instar larval eye imaginal discs were dissected for RNA extraction and hybridization on Affymetrix microarray. Flies from each genotype were used (I: eyeless-Gal4; UAS-lacZ, UAS-lacZ, II: ey-GAL4; UAS-lacZ, UAS-simj, III: ey-Gal4; UAS-vg, UAS-lacZ, IV: ey-Gal4 UAS-vg; UAS-simj. Two biologic replicates were obtained for each.
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| Contributor(s) |
Masuko K, Kurata S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 22, 2019 |
| Last update date |
Jan 02, 2021 |
| Contact name |
Keita Masuko |
| E-mail(s) |
ke.masuko@gmail.com
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| Organization name |
Tohoku University
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| Department |
Pharmaceutical Science
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| Street address |
Aoba 6-3, Aramaki, Aoba-ku,
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| City |
Sendai |
| ZIP/Postal code |
980-8578 |
| Country |
Japan |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (8)
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| GSM3573720 |
eye disc of ey>lacZ, lacZ, biologic rep 1 |
| GSM3573721 |
eye disc of ey>lacZ, lacZ, biologic rep 2 |
| GSM3573722 |
eye disc of ey>vg, lacZ, biologic rep 1 |
| GSM3573723 |
eye disc of ey>vg, lacZ, biologic rep 2 |
| GSM3573724 |
eye disc of ey>lacZ, simj, biologic rep 1 |
| GSM3573725 |
eye disc of ey>lacZ, simj, biologic rep 2 |
| GSM3573726 |
eye disc of ey>vg, simj, biologic rep 1 |
| GSM3573727 |
eye disc of ey>vg, simj, biologic rep 2 |
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| Relations |
| BioProject |
PRJNA516350 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125425_RAW.tar |
14.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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