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| Status |
Public on Sep 26, 2019 |
| Title |
Bap1 loss leads to decreased expression of lineage specific commitment genes, and increased expression of pluripotency genes [RNA-seq] |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed morpholino-mediated knockdown of Bap1 protein expression in Xenopus laevis developing embryos, and analyzed gene expression at stage 12. We find that inhibition of Bap1 leads to decrease in lineage specific commitment genes, and increased expression of pluripotency genes.
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| |
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| Overall design |
RNA profiles of stage 12 embryos injected with either Ctrl morpholino (CtrlMO) or mopholino targeting Bap1 (Bap1MO), in triplicate, using Illumina Nextseq500.
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| |
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| Contributor(s) |
Kuznetsov JN, Field MG, Durante MA |
| Citation(s) |
31555735 |
| |
| Submission date |
Feb 14, 2019 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Michael Durante |
| E-mail(s) |
mdurante@med.miami.edu
|
| Phone |
2393575338
|
| Organization name |
University of Miami Miller School of Medicine
|
| Department |
Ophthalmology
|
| Lab |
Harbour
|
| Street address |
1501 NW 10th Ave., Ste 832
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21248 |
Illumina NextSeq 500 (Xenopus laevis) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE126599 |
Loss of Bap1 in Xenopus laevis embryos |
|
| Relations |
| BioProject |
PRJNA522475 |
| SRA |
SRP185947 |
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