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| Status |
Public on Dec 18, 2008 |
| Title |
Laser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus2.0 GeneChips. In the first instance, we used the dataset to compare the neuronal and endothelial data, in order to demonstrate that the predicted differences between cell types could be detected using this methodology.
Keywords: cell type comparison, laser capture microdissection
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| Overall design |
The dataset consists of endothelial cell samples from 18 individuals and neuronal samples from an overlapping population of 18 individuals.
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| Contributor(s) |
Harris LW, Wayland MT, Ryan MM, Lan MJ, Giger T, Lockstone H, Wang L, Mimmack ML, Kotter M, Wuethrich I, Bahn S |
| Citation(s) |
19088852 |
| |
| Submission date |
Sep 05, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Laura Wiseman Harris |
| Organization name |
University of Cambridge
|
| Department |
Insitute of Chemical Engineering and Biotechnology
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| Street address |
Tennis Court Road
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| City |
Cambridge |
| ZIP/Postal code |
CB22 4RA |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA112675 |
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