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| Status |
Public on Jan 21, 2020 |
| Title |
Genes expressed in the pleuropodia from embryos of the locust Schistocerca gregaria (Insecta, Orthoptera). |
| Organism |
Schistocerca gregaria |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-seq was used to study the genes expressed in peculiar glandular organs that transiently appear in insect embryos and are called the pleuropodia. The locust Schistocerca gregaria (Orthoptera) was used as a model. The purpose of the study is to identify the function of these organs. Our results suport the hypothesis (Slifer, 1937) that the pleuropodia secrete enzymes that digest the serosal cuticle before hatching. Additionally, we found that the pleuropodia may also have other functions, such as in embryonic immunity. The pleuropodia are peculiarly modified limbs. We show that in their early stages both legs and pleuropodia share a similar genetic landscape, but as the appendages become more morphologically diverse they became also more diverse genetically.
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| Overall design |
Pleuropodia and legs of the third pair ("T3") at an equal age were compared. To identify DEGs upregulated in the mature pleuropodia that have morphological markers of active (secreting) organs samples from stages 10, 11 and 12 days were merged (legs and pleuropodia separately).
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| Contributor(s) |
Konopova B, Buchberger E, Crisp A |
| Citation |
Konopová B, Buchberger E and Crisp A. Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch. Front Zool 2020;17:4. doi:10.1186/s12983-019-0349-2
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| BioProject |
PRJNA524786 |
| |
| Submission date |
Mar 15, 2019 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Barbora Konopova |
| E-mail(s) |
barbora.konopova@entu.cas.cz
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| Organization name |
Biology Centre CAS
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| Department |
Institute of Entomology
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| Lab |
Laboratory of Insect Physiology
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| Street address |
Branisovska 31
|
| City |
Ceske Budejovice |
| ZIP/Postal code |
37005 |
| Country |
Czech Republic |
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| Platforms (2) |
| GPL16114 |
Illumina HiSeq 2000 (Schistocerca gregaria) |
| GPL26311 |
Illumina Genome Analyzer IIx (Schistocerca gregaria) |
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| Samples (20)
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| Relations |
| SRA |
SRP188106 |
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