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| Status |
Public on Sep 20, 2008 |
| Title |
Gene expression profiles of lymphatic endothelial cells after knockdown of Prox1 and/or NR2F2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression profiles of primary lymphatic endothelial cells (LECs) isolated from human foreskin were analyzed after siRNA-mediated knockdown of control (firefly luciferase), Prox1, NR2F2 or Prox1/NR2F2 for 48 hours.
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| Overall design |
Passage five human lymphatic endothelial cells (LECs) were cultured on fibronectin (10 μg/ml)-coated plates in a complete media (EBM, 20% FBS supplemented with 10 μg/ml hydrocortisone acetate, 25 ug/ml cAMP and antibiotics). LECs were harvested and electorporated with siRNA duplexes for 48 hours with siRNA duplexes against either firefly luciferase(control), Prox1, NR2F2 or Prox1/NR2F2. Total RNA was purified using Tri-reagent and was subjected to microarray analysis.
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| Contributor(s) |
Lee S, Hong Y |
| Citation(s) |
18815287, 22719258 |
| |
| Submission date |
Sep 18, 2008 |
| Last update date |
Aug 27, 2019 |
| Contact name |
Young-Kwon Hong |
| Organization name |
University of Southern California
|
| Street address |
1450 Biggy St. NRT6501
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA111071 |
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