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| Status |
Public on Sep 05, 2019 |
| Title |
X chromosome domain architecture regulates Caenorhabditis elegans lifespan but not dosage compensation [RNA-seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mechanisms establishing higher-order chromosome structures and their roles in gene regulation are elusive. We analyzed chromosome architecture during nematode X-chromosome dosage compensation, which represses transcription via a dosage-compensation condensin complex (DCC) that binds hermaphrodite Xs and establishes megabase-size topologically associating domains (TADs). We show that DCC binding at high-occupancy sites (rex sites) defines eight TAD boundary locations. Single rex deletions disrupted boundaries, and single insertions created new boundaries, demonstrating one rex site is necessary and sufficient for DCC-dependent boundary formation. Deleting eight rex sites (8rexΔ) recapitulated TAD structure of DCC mutants, permitting analysis when chromosome-wide domain architecture was disrupted but most DCC binding remained. 8rexΔ animals exhibited no changes in X expression and lacked dosage-compensation mutant phenotypes. Hence, TAD boundaries are neither the cause nor consequence of gene repression during dosage compensation. Abrogating TAD structure did, however, reduce thermotolerance, accelerate aging, and shorten lifespan, implicating chromosome architecture in regulating stress responses and aging.
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| Overall design |
We performed in RNA-seq on five replicates of wild-type and 8rexΔ embryos and three replicates of DCC mutant embryos. For each replicate, worms were grown and embryos collected for all genotypes in parallel. Gene expression in 8rexΔ and DCC mutant is compared to wild-type expression.
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| Contributor(s) |
Anderson EC, Frankino PA, Higuchi-Sanabria R, Yang Q, Bian Q, Podshivolova K, Shin A, Kenyon C, Dillin A, Meyer BJ |
| Citation(s) |
31491396 |
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| Submission date |
Mar 19, 2019 |
| Last update date |
Dec 05, 2019 |
| Contact name |
Elphege P Nora |
| E-mail(s) |
elphege.nora@ucsf.edu
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| Organization name |
UCSF
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| Department |
CVRI
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| Lab |
Nora
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| Street address |
555 Mission Bay Blvd S
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL22765 |
Illumina HiSeq 4000 (Caenorhabditis elegans) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE128568 |
X chromosome domain architecture regulates Caenorhabditis elegans lifespan but not dosage compensation |
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| Relations |
| BioProject |
PRJNA528111 |
| SRA |
SRP188887 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE128566_WT_8rexDeletion_DCCmutant_RNAseq.txt.gz |
884.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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