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| Status |
Public on Oct 10, 2019 |
| Title |
RNA-seq of human foreskin fibroblast cells lacking RB and/or p130 after doxorubicin treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used human foreskin fibroblast cells with CRISPR-Cas9 mediated knockout of RB1 and p130 (RBL2) (Control, sgRB1, sgP130, sgRB1+sg130) and measured gene expression changes after doxorubicin treatmnet (24 hr, 350 nM).
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| Overall design |
Control, sgP130, sgRB1, and sgRB1+sgP130 HFFs were contact arrested for 48 hours. Cells were released from contact inhibition by splitting into complete media containing 0 nM or 350 nM doxorubicin. Cells were collected after 24 hours and RNA was harvested for sequencing. Experiment was completed with two biological replicates for each cell line in each condition resulting in 16 samples run in a single lane for RNAseq.
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| Contributor(s) |
Schade AE, Fischer M, DeCaprio JA |
| Citation(s) |
31667499 |
| |
| Submission date |
Mar 22, 2019 |
| Last update date |
Jan 09, 2020 |
| Contact name |
Amy E Schade |
| E-mail(s) |
aschade@bwh.harvard.edu
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| Organization name |
Brigham and Women's Hospital
|
| Department |
Medicine
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| Lab |
Karen Cichowski
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| Street address |
77 Avenue Louis Pasteur
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA528619 |
| SRA |
SRP189155 |
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