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| Status |
Public on Mar 21, 2022 |
| Title |
Transcriptome analysis of PRRSV Nsp1 overexpressed 3D4/31 cells |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study investigated the immunological function of PRRSV Nsp1 by ectopic expression of PRRSV Nsp1 in 3D4/31 cell line. Identifying the functional role of PRRSV Nsp1 associated with host cell modulation may provide better knowledge about the pathogenesis of PRRS (Porcine reproductive and respiratory syndrome).
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| Overall design |
A macrophage cell line, 3D4/31, was transfected with pBabepuro (Mock), PRRSV NSP1-pBabepuro (PRRSV NSP1), PRRSV NSP1 alpha-pBabepuro (PRRSV NSP1a), or PRRSV NSP1 beta-pBabepuro (PRRSV NSP1b). Cloned PRRSV NSP1, NSP1 alpha, and NSP1 beta is derived from PRRSV VR2332 (GenBank accession U87392) (Cheng Song et al, Virology, 2010). To identify differentially mRNAs in the PRRSV NSP1, PRRSV NSP1 alpha, PRRSV NSP1 beta transfectants and mock, three cDNA libraries from each transfectant group were generated and sequenced using the Illumina NovaSeq 6000 sequencer.
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| Contributor(s) |
Park IB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 27, 2019 |
| Last update date |
Mar 21, 2022 |
| Contact name |
In byung Park |
| Organization name |
Korea University
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| Street address |
145, Anam-ro, Seongbuk-gu, Seoul
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| City |
Seoul |
| ZIP/Postal code |
02841 |
| Country |
South Korea |
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| Platforms (1) |
| GPL26351 |
Illumina NovaSeq 6000 (Sus scrofa) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA529390 |
| SRA |
SRP189594 |
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