|
| Status |
Public on Apr 12, 2019 |
| Title |
Disease modelling of core pre-mRNA splicing factor haploinsufficiency |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated a human EFTUD2 knockdown cell line using a CRISPR cas9 nickase strategy to investigate the effects of decreased expression of core spliceosome components on cell characteristics and global transcriptome expression/splicing patterns
|
| |
|
| Overall design |
6 biological replicates of WT or CRISPR knock-down cells were generated and analysed by RNA-Seq
|
| |
|
| Contributor(s) |
Wood KA, Rowlands CF, Thomas HB, Buczek WA, Briggs TA, Hubbard SJ, Newman WG, O'Keefe RT |
| Citation(s) |
31304552 |
| |
| Submission date |
Apr 10, 2019 |
| Last update date |
Jul 23, 2019 |
| Contact name |
Huw Batten Thomas |
| E-mail(s) |
huw.thomas@manchester.ac.uk
|
| Organization name |
University of Manchester
|
| Department |
Biological Sciences
|
| Lab |
Michael Smith Building
|
| Street address |
Dover Street
|
| City |
Manchester |
| State/province |
Greater Manchester |
| ZIP/Postal code |
M13 9PT |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA532422 |
| SRA |
SRP192094 |
Less...
More...