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| Status |
Public on Jun 20, 2021 |
| Title |
Genome-wide maps of nuclear lamina interactions in AML12 cells upon G9a/GLP inhibition [DamID] |
| Organism |
Mus musculus |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
In differentiated cells, inhibition of methyltransferases G9a and GLP eliminated H3K9me2 predominantly at A-type genomic compartments, and the remaining H3K9me2 marks were strongly associated with B-type compartments and lamina-associated domains (LADs). However, inhibition of G9a/GLP in mouse embryonic stem cells (ESCs) removed most of the H3K9me2 modifications in both A and B compartments. Furthermore, chemical inhibition of G9a/GLP in mouse hepatocytes decreased chromatin-nuclear lamina (NL) interactions, increased the degree of genomic compartmentalization and the boundary strength of topologically associating domains (TADs) between A and B compartments, and altered the expression of hundreds of genes that were associated with alterations of chromatin structure.
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| Overall design |
LAMINB1 DamID data in DMSO and UNC0638 treated AML12 cells.
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| Citation missing |
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| |
| Submission date |
Jun 18, 2019 |
| Last update date |
Jun 21, 2021 |
| Contact name |
Bo Wen |
| E-mail(s) |
bowen75@fudan.edu.cn
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| Organization name |
Fudan University
|
| Street address |
130 Dongan Rd.
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| City |
ShangHai |
| ZIP/Postal code |
200032 |
| Country |
China |
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| Platforms (1) |
| GPL10448 |
Agilent-027414 SurePrint G3 Mouse CGH Microarray 1x1M |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA549523 |
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