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| Status |
Public on Jul 23, 2019 |
| Title |
Real-time quantitative PCR analysis of human gingiva epithelial cells exposed to microbial biofilms |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
An organotypic gingiva model RHG was constructed by immortalized human gingiva cell lines (keratinocytes and fibroblasts) in vitro. The RHG model was exposed to either control medium or one of the three multi-species oral biofilms (commensal, gingivitis and cariogenic) for 24 hours. We used Qiagen RT² Profiler PCR array to analyse the expression of genes which are related to Toll-like receptor signaling pathway from the unexposed (control) and differently exposed (test) keratinocyte layer of the RHG model. Reference for RHG model and oral biofilms culture: J. K. Buskermolen et al., J Dent Res 97 (2018): 201-208. doi: 10.1177/0022034517729998.
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| Overall design |
qPCR gene expression profiling. For each exposure condition, three (commensal, gingivitis) or two (cariogenic) replicates of RHG samples were used. Equal amount total RNA from each sample was processed to gene expression analysis.
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| Contributor(s) |
Gibbs S |
| Citation(s) |
31448244 |
| |
| Submission date |
Jun 27, 2019 |
| Last update date |
Oct 22, 2019 |
| Contact name |
Susan Gibbs |
| E-mail(s) |
s.gibbs@vumc.nl
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| Organization name |
Academic Centre for Dentistry Amsterdam
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| Department |
Oral Cell Biology
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| Street address |
Gustav Mahlerlaan 3004
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| City |
Amsterdam |
| ZIP/Postal code |
1081LA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL24875 |
SABiosciences RT² Profiler™ PCR Array Human Toll-Like Receptor Signaling Pathway [PAHS-018Z] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA551435 |
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