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Series GSE13396 Query DataSets for GSE13396
Status Public on Nov 01, 2008
Title Rhinovirus-induced modulation of gene expression in bronchial epithelial cells from subjects with asthma
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human Rhinovirus (HRV) infection can trigger exacerbations of asthma. Understanding of the mechanisms provoking airway inflammation and remodeling in asthma, as well as the pathogenic mechanisms of HRV infection and its association with asthma exacerbations, may offer significant opportunities for improved disease management. Genome-wide expression analysis of HRV type 1A-infected primary bronchial epithelial (PBE) cells from normal and asthmatic donors was performed to determine whether asthma is associated with a unique pattern of gene expression after HRV infection in vitro.

Keywords: response to rhinovirus infection
 
Overall design Frozen stocks of human PBE cells obtained from the bronchial brushings of six normal and five asthmatic individuals were grown in vitro in Bronchial Epithelial Growth Media (BEGM, Lonza). Subconfluent monolayers of PBE cells were infected with purified and concentrated minor group HRV serotype 1A at multiplicity of infection of 10 plaque forming units (pfu) per cell or mock-infected with growth media alone. Cells were lysed 16 hours post infection (p.i.).
Isolated total RNAs were analyzed using the Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays (Samples S7-S18) or Affymetrix Human HG-Focus arrays samples (Samples S1-S6).
Samples S1-S6 and Samples S7-S18 were normalized by RMA using different methods.
 
Contributor(s) Bochkov YA, Hanson KM, Keles S, Brockman-Schneider RA, Gern JE
Citation(s) 19710636
Submission date Oct 29, 2008
Last update date Mar 25, 2019
Contact name Yury A Bochkov
E-mail(s) yabochkov@wisc.edu
Phone 608-263-8553
Organization name University Of Wisconsin-Madison
Department Pediatrics
Street address 600 Highland Ave, K4/945 CSC
City Madison
State/province WI
ZIP/Postal code 53792-9988
Country USA
 
Platforms (2)
GPL201 [HG-Focus] Affymetrix Human HG-Focus Target Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (34)
GSM338272 mock-infected, asthmatic S10 (U133 Plus 2.0 Array)
GSM338273 mock-infected, asthmatic S12 (U133 Plus 2.0 Array)
GSM338274 mock-infected, asthmatic S16 (U133 Plus 2.0 Array)
Relations
BioProject PRJNA109753

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE13396_RAW.tar 114.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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